Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS(Da)
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E6636Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 8ng/ml | 1.924 | 1.938 | 1.902 |
| 1.951 | |||
| 4ng/ml | 1.027 | 1.065 | 1.03 |
| 1.103 | |||
| 2ng/ml | 0.445 | 0.49 | 0.454 |
| 0.535 | |||
| 1ng/ml | 0.318 | 0.277 | 0.241 |
| 0.236 | |||
| 0.5ng/ml | 0.147 | 0.15 | 0.115 |
| 0.153 | |||
| 0ng/ml | 0.038 | 0.035 | 0 |
| 0.033 |
Precision
We measured random samples of E6636Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 2.85 | 0.1 | 3.65 |
| Intra-Assay | 2 | 18 | 5.33 | 0.14 | 2.62 |
| Intra-Assay | 3 | 18 | 1.72 | 0.08 | 4.79 |
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