Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
Background
N/A
UniProt accession
MASS（Da）
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E6679Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 800ng/L | 1.842 | 1.909 | 1.875 |
| 1.975 | |||
| 400ng/L | 1.031 | 1.062 | 1.028 |
| 1.093 | |||
| 200ng/L | 0.412 | 0.441 | 0.408 |
| 0.47 | |||
| 100ng/L | 0.258 | 0.249 | 0.216 |
| 0.24 | |||
| 50ng/L | 0.17 | 0.169 | 0.136 |
| 0.168 | |||
| 0ng/L | 0.037 | 0.034 | 0 |
| 0.03 |
Precision
We measured random samples of E6679Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 367.39 | 15.31 | 4.17 |
| Intra-Assay | 2 | 18 | 745.36 | 26.15 | 3.51 |
| Intra-Assay | 3 | 18 | 240.54 | 13.16 | 5.47 |
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