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Home > Product > ELISA Kit > Human Myosin Light Chain 3, MYL3 ELISA Kit

Human Myosin Light Chain 3, MYL3 ELISA Kit

Human Myosin Light Chain 3, MYL3 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 12.93ng/L

Detection range: 20-4500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 12.93ng/L

Detection range: 20-4500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Myosin Light Chain 3

Code

E6684Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

12.93ng/L

Detection range

20-4500ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Myosin essential light chain (ELC), ventricular/cardiac isoform is a protein that in humans is encoded by the MYL3 gene. This cardiac ventricular/slow skeletal ELC isoform is distinct from that expressed in fast skeletal muscle (MYL1) and cardiac atrial muscle (MYL4). Ventricular ELC is part of the myosin molecule and is important in modulating cardiac muscle contraction. Studies have provided evidence for ELC as modulator of myosin crossbridge kinetics. Treating cardiac myofibrils with the lysine-rich N-terminal peptide (amino acids 5-14) evoked a supramaximal increase in cardiac myofibrillar MgATPase activity at submaximal calcium concentrations, and further studies demonstrated that this region of ELC modulates the affinity of myosin for actin.

UniProt accession

MASS(Da)

21,932

GeneID

4634

Synonyms

Cardiac myosin light chain 1;CMH8;CMLC1;MLC1SB;MLC1V;MLC-lV/sb;MYL3;Myosin light chain 1, slow-twitch muscle B/ventricular isoform;Myosin light chain 3;Ventricular myosin alkali light chain;Ventricular myosin light chain 1;Ventricular/slow twitch myosin alkali light chain;VLC1;VLCl

Gene names

MYL3

Research area

Signal Transduction

Target protein

MYL3

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E6684Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
2400ng/L 1.939 1.907 1.874
1.874
1200ng/L 1.126 1.071 1.039
1.017
600ng/L 0.59 0.502 0.469
0.413
300ng/L 0.272 0.22 0.187
0.168
150ng/L 0.148 0.136 0.103
0.125
0ng/L 0.042 0.033 0
0.024

Precision

We measured random samples of E6684Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 623.78 28.99 4.65
Intra-Assay 2 18 1788.69 50.43 2.82
Intra-Assay 3 18 933.82 38.42 4.11

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E6684Hu

    $320.00

  • 96T

    E6684Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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