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Home > Product > ELISA Kit > Human Cytochrome B-C1 Complex Subunit 2, UQCRC2 ELISA Kit

Human Cytochrome B-C1 Complex Subunit 2, UQCRC2 ELISA Kit

Human Cytochrome B-C1 Complex Subunit 2, UQCRC2 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 5.1ng/L

Detection range: 10-2000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 5.1ng/L

Detection range: 10-2000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Cytochrome b-c1 complex subunit 2

Code

E6753Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

5.1ng/L

Detection range

10-2000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Component of the ubiquinol-cytochrome c oxidoreductase, a multisubunit transmembrane complex that is part of the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. The cytochrome b-c1 complex catalyzes electron transfer from ubiquinol to cytochrome c, linking this redox reaction to translocation of protons across the mitochondrial inner membrane, with protons being carried across the membrane as hydrogens on the quinol. In the process called Q cycle, 2 protons are consumed from the matrix, 4 protons are released into the intermembrane space and 2 electrons are passed to cytochrome c (By similarity). The 2 core subunits UQCRC1/QCR1 and UQCRC2/QCR2 are homologous to the 2 mitochondrial-processing peptidase (MPP) subunits beta-MPP and alpha-MPP respectively, and they seem to have preserved their MPP processing properties (By similarity). May be involved in the in situ processing of UQCRFS1 into the mature Rieske protein and its mitochondrial targeting sequence (MTS)/subunit 9 when incorporated into complex III (Probable).

UniProt accession

MASS(Da)

48,443

GeneID

7385

Synonyms

Complex III subunit 2;Core protein II;Cytochrome b-c1 complex subunit 2, mitochondrial;MC3DN5;QCR2;Ubiquinol-cytochrome-c reductase complex core protein 2;UQCR2;UQCRC2

Gene names

UQCRC2

Research area

Signal Transduction; Cancer; Metabolism

Target protein

UQCRC2

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E6753Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1200ng/L 2.259 2.245 2.2
2.23
600ng/L 1.382 1.363 1.318
1.344
300ng/L 0.68 0.745 0.7
0.809
150ng/L 0.54 0.497 0.452
0.455
75ng/L 0.245 0.283 0.238
0.321
0ng/L 0.04 0.045 0
0.05

Precision

We measured random samples of E6753Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 889.04 36.24 4.08
Intra-Assay 2 18 276.89 21.23 7.67
Intra-Assay 3 18 535.36 23.01 4.3

Protocols

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Associated products

  • Size

    Code

    Price

  • 48T

    E6753Hu

    $320.00

  • 96T

    E6753Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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