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Home > Product > ELISA Kit > Human Nadh Dehydrogenase, Ubiquinone, Iron-sulfur Protein 7, NDUFS7 ELISA Kit

Human Nadh Dehydrogenase, Ubiquinone, Iron-sulfur Protein 7, NDUFS7 ELISA Kit

Human Nadh Dehydrogenase, Ubiquinone, Iron-sulfur Protein 7, NDUFS7 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 5.22ng/L

Detection range: 10-2000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 5.22ng/L

Detection range: 10-2000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

NADH dehydrogenase [ubiquinone] iron-sulfur protein 7

Code

E6755Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

5.22ng/L

Detection range

10-2000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial, also knowns as NADH-ubiquinone oxidoreductase 20 kDa subunit, Complex I-20kD (CI-20kD), or PSST subunit is an enzyme that in humans is encoded by the NDUFS7 gene. The PSST subunit encoded by the NDUSF7 gene is one of over 40 subunits involved in the transfer of electrons from NADH to ubiquinone. Specifically, it is thought that the PSST subunit directly couples electron transfer between the iron-sulfur cluster N2 and ubiquinone, along with ubiquinone-binding ND1.Functional evidence for the importance of PSST has been garnered from mutational studies in the obligate aerobic yeast, Yarrow lipolytic, which elucidated a central role in proton translocation that was reduced in mutant forms of the subunit.

UniProt accession

MASS(Da)

23,564

GeneID

374291

Synonyms

CI-20;CI-20KD;Complex I-20kD;MC1DN3;MY017;NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial;NADH-ubiquinone oxidoreductase 20 kDa subunit;NDUFS7;PSST;PSST subunit

Gene names

NDUFS7

Research area

Tags & Cell Markers; Signal Transduction; Neuroscience; Metabolism

Target protein

NDUFS7

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E6755Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1200ng/L 1.97 1.908 1.882
1.847
600ng/L 1.019 0.951 0.925
0.884
300ng/L 0.643 0.605 0.578
0.567
150ng/L 0.143 0.179 0.153
0.215
75ng/L 0.118 0.114 0.087
0.109
0ng/L 0.026 0.027 0
0.027

Precision

We measured random samples of E6755Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 1044.44 26.95 2.58
Intra-Assay 2 18 550.27 23.73 4.31
Intra-Assay 3 18 289.31 15.51 5.36

Protocols

References

Customer reviews and Q&As

No reviews
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Associated products

  • Size

    Code

    Price

  • 48T

    E6755Hu

    $320.00

  • 96T

    E6755Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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