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Home > Product > ELISA Kit > Human Coatomer Subunit Alpha, COPA ELISA Kit

Human Coatomer Subunit Alpha, COPA ELISA Kit

Human Coatomer Subunit Alpha, COPA ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.094ng/ml

Detection range: 0.2-60ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.094ng/ml

Detection range: 0.2-60ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Coatomer subunit Alpha

Code

E6842Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.094ng/ml

Detection range

0.2-60ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors (By similarity). Xenin stimulates exocrine pancreatic secretion. It inhibits pentagastrin-stimulated secretion of acid, to induce exocrine pancreatic secretion and to affect small and large intestinal motility. In the gut, xenin interacts with the neurotensin receptor.

UniProt accession

MASS(Da)

138,346

GeneID

1314

Synonyms

AILJK;Alpha-coat protein;alpha-COP;Coatomer subunit alpha;COPA;HEPCOP;HEP-COP;Proxenin];Xenin;Xenopsin-related Peptide

Gene names

COPA

Research area

Signal Transduction

Target protein

COPA

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E6842Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
32ng/ml 2.193 2.071 2.044
1.948
16ng/ml 1.398 1.456 1.429
1.514
8ng/ml 0.723 0.64 0.614
0.557
4ng/ml 0.44 0.443 0.417
0.446
2ng/ml 0.167 0.16 0.133
0.153
0ng/ml 0.031 0.027 0
0.022

Precision

We measured random samples of E6842Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 18.35 0.7 3.83
Intra-Assay 2 18 4.03 0.18 4.52
Intra-Assay 3 18 8.97 0.43 4.83

Protocols

References

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 48T

    E6842Hu

    $320.00

  • 96T

    E6842Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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