Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS(Da)
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E7304Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 1000ng/L | 2.279 | 2.18 | 2.131 |
| 2.08 | |||
| 500ng/L | 1.14 | 1.223 | 1.175 |
| 1.307 | |||
| 250ng/L | 0.685 | 0.687 | 0.638 |
| 0.689 | |||
| 125ng/L | 0.459 | 0.438 | 0.389 |
| 0.417 | |||
| 62.5ng/L | 0.256 | 0.24 | 0.192 |
| 0.225 | |||
| 0ng/L | 0.052 | 0.049 | 0 |
| 0.045 |
Precision
We measured random samples of E7304Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 348.77 | 17.47 | 5.01 |
| Intra-Assay | 2 | 18 | 559.7 | 20.13 | 3.6 |
| Intra-Assay | 3 | 18 | 179.58 | 12 | 6.68 |
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