Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS(Da)
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E7319Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 2400ng/L | 2.185 | 2.197 | 2.155 |
| 2.208 | |||
| 1200ng/L | 1.182 | 1.188 | 1.147 |
| 1.195 | |||
| 600ng/L | 0.706 | 0.727 | 0.685 |
| 0.748 | |||
| 300ng/L | 0.42 | 0.394 | 0.353 |
| 0.369 | |||
| 150ng/L | 0.203 | 0.199 | 0.157 |
| 0.195 | |||
| 0ng/L | 0.037 | 0.042 | 0 |
| 0.046 |
Precision
We measured random samples of E7319Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 955.33 | 44.3 | 4.64 |
| Intra-Assay | 2 | 18 | 1336.27 | 37.34 | 2.79 |
| Intra-Assay | 3 | 18 | 590.35 | 31.76 | 5.38 |
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