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Home > Product > ELISA Kit > Human Bcl-2-like Protein 1, BCLX ELISA Kit

Human Bcl-2-like Protein 1, BCLX ELISA Kit

Human Bcl-2-like Protein 1, BCLX ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.13ng/mL

Detection range: 0.3-90ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.13ng/mL

Detection range: 0.3-90ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Bcl-2-Like Protein 1

Code

E4035hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.13ng/mL

Detection range

0.3-90ng/mL

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Potent inhibitor of cell death. Inhibits activation of caspases. Appears to regulate cell death by blocking the voltage-dependent anion Channel (VDAC) by binding to it and preventing the release of the caspase activator, CYC1, from the mitochondrial membrane. Also acts as a regulator of G2 checkpoint and progression to cytokinesis during mitosis. Isoform Bcl-X(L) also regulates presynaptic plasticity, including neurotransmitter release and recovery, number of axonal mitochondria as well as size and number of synaptic vesicle clusters. During synaptic stimulation, increases ATP availability from mitochondria through regulation of mitochondrial membrane ATP Synthase F(1)F(0) activity and regulates endocytic vesicle retrieval in hippocampal neurons through association with DMN1L and stimulation of its GTPase activity in synaptic vesicles. May attenuate inflammation impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release (PubMed: 17418785). Isoform Bcl-X(S) promotes apoptosis.

UniProt accession

MASS(Da)

26,049

GeneID

598

Synonyms

Apoptosis regulator Bcl-X;BCL2L1;Bcl2-L-1;Bcl-2-like protein 1

Gene names

BCL2L1

Research area

Cell Biology

Target protein

BCL2L1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of Human Bcl-2-like Protein 1, BCLX ELISA Kit are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
48ng/mL 2.296 2.275 2.225
2.255
24ng/mL 1.131 1.265 1.215
1.4
12ng/mL 0.824 0.764 0.713
0.704
6ng/mL 0.456 0.46 0.41
0.465
3ng/mL 0.195 0.215 0.165
0.236
0ng/mL 0.055 0.051 0
0.046

Precision

We measured random samples of E4035hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 19 0.94 5
Intra-Assay 2 18 35.4 1.36 3.8
Intra-Assay 3 18 4.9 0.28 5.6

Protocols

References

Customer reviews and Q&As

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Product size

Price

  • 48T

    $320.00

  • 96T

    $458.00

Add to Cart

Lead time: Within one week

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