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Home > Product > ELISA Kit > Human Cystic Fibrosis Transmembrane Conductance Regulator, CFTR ELISA Kit

Human Cystic Fibrosis Transmembrane Conductance Regulator, CFTR ELISA Kit

Human Cystic Fibrosis Transmembrane Conductance Regulator, CFTR ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 9.28ng/L

Detection range: 20-3800ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 9.28ng/L

Detection range: 20-3800ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Cystic Fibrosis Transmembrane Conductance Regulator

Code

E4110hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

9.28ng/L

Detection range

20-3800ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Epithelial ion Channel that plays an important role in the regulation of epithelial ion and water transport and fluid homeostasis (PubMed: 26823428). Mediates the transport of chloride ions across the cell membrane (PubMed: 10792060, PubMed: 11524016, PubMed: 11707463, PubMed: 12519745, PubMed: 15010471, PubMed: 12588899, PubMed: 17036051, PubMed: 19398555, PubMed: 19621064, PubMed: 22178883, PubMed: 25330774, PubMed: 1712898, PubMed: 8910473, PubMed: 9804160, PubMed: 12529365, PubMed: 17182731, PubMed: 26846474, PubMed: 28087700). Channel activity is coupled to ATP hydrolysis (PubMed: 8910473). The ion Channel is also permeable to HCO(3-); selectivity depends on the extracellular chloride concentration (PubMed: 15010471, PubMed: 19019741). Exerts its function also by modulating the activity of other ion Channels and transporters (PubMed: 12403779, PubMed: 22178883, PubMed: 22121115, PubMed: 27941075). Plays an important role in airway fluid homeostasis (PubMed: 16645176, PubMed: 19621064, PubMed: 26823428). Contributes to the regulation of the pH and the ion content of the airway surface fluid layer and thereby plays an important role in defense against pathogens (PubMed: 14668433, PubMed: 16645176, PubMed: 26823428). Modulates the activity of the epithelial sodium Channel (ENaC) complex, in part by regulating the cell surface expression of the ENaC complex (PubMed: 17434346, PubMed: 27941075, PubMed: 17182731). Inhibits the activity of the ENaC Channel containing subunits SCNN1A, SCNN1B and SCNN1G (PubMed: 17182731). Inhibits the activity of the ENaC Channel containing subunits SCNN1D, SCNN1B and SCNN1G, but not of the ENaC Channel containing subunits SCNN1A, SCNN1B and SCNN1G (PubMed: 27941075). May regulate bicarbonate secretion and salvage in epithelial cells by regulating the transporter SLC4A7 (PubMed: 12403779). Can inhibit the chloride Channel activity of ANO1 (PubMed: 22178883). Plays a role in the chloride and bicarbonate homeostasis during sperm epididymal maturation and capacitation (PubMed: 19923167, PubMed: 27714810).

UniProt accession

MASS(Da)

168,142

GeneID

1080

Synonyms

ATP-binding cassette sub-family C member 7;cAMP-dependent chloride Channel;CFTR;Channel conductance-controlling ATPase;Cystic fibrosis transmembrane conductance regulator

Gene names

CFTR

Research area

Signal Transduction

Target protein

CFTR

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E4110hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
2000ng/L 2.141 2.157 2.101
2.174
1000ng/L 1.555 1.538 1.482
1.521
500ng/L 1.175 1.147 1.091
1.12
250ng/L 0.742 0.698 0.642
0.654
125ng/L 0.442 0.445 0.389
0.448
0ng/L 0.049 0.056 0
0.063

Precision

We measured random samples of E4110hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E4110hu

    $320.00

  • 96T

    E4110hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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