Human Nadphoxidase 4, NOX4 ELISA Kit

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Home > Product > ELISA Kit > Human Nadphoxidase 4, NOX4 ELISA Kit

Human Nadphoxidase 4, NOX4 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 2.36ng/L

Detection range: 5-1500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 2.36ng/L

Detection range: 5-1500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Nadph Oxidase 4

Code

E4156hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

2.36ng/L

Detection range

5-1500ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Constitutive NADPH oxidase which generates superoxide intracellularly upon formation of a complex with CYBA/p22phox. Regulates signaling cascades probably through phosphatases inhibition. May function as an oxygen sensor regulating the KCNK3/TASK-1 potassium Channel and HIF1A activity. May regulate insulin signaling cascade. May play a role in apoptosis, bone resorption and lipolysaccharide-mediated activation of NFKB. May produce superoxide in the nucleus and play a role in regulating gene expression upon cell stimulation. Isoform 3 is not functional. Isoform 5 and isoform 6 display reduced activity. [Isoform 4]: Involved in redox signaling in vascular cells. Constitutively and NADPH-dependently generates reactive oxygen species (ROS). Modulates the nuclear activation of ERK1/2 and the ELK1 transcription factor, and is capable of inducing nuclear DNA damage. Displays an increased activity relative to isoform 1.

UniProt accession

Q9NPH5

MASS（Da）

66,932

GeneID

50507

Synonyms

Kidney oxidase-1;Kidney superoxide-producing NADPH oxidase;KOX-1;NADPH oxidase 4;NOX 4;NOX4;Renal NAD(PH-oxidase

Gene names

NOX4

Research area

Cardiovascular

Target protein

NOX4

Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Protocol data

These standard curves of E4156hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
800ng/L	2.376	2.241	2.189
	2.106
400ng/L	1.229	1.288	1.236
	1.347
200ng/L	0.767	0.771	0.719
	0.775
100ng/L	0.306	0.347	0.295
	0.387
50ng/L	0.158	0.172	0.12
	0.187
0ng/L	0.051	0.052	0
	0.053

Precision

We measured random samples of E4156hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	268.4	14.91	5.6
Intra-Assay	2	18	399.6	14.02	3.5
Intra-Assay	3	18	103.4	5.75	5.6

Protocols

> Protocol Booklet

References

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Associated products

Datasheet

Size

Code

Price

48T

E4156hu

$320.00
96T

E4156hu

$458.00

Add to Cart

Lead time: Within two weeks

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