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Home > Product > ELISA Kit > Human Selenoprotein N, SELN ELISA Kit

Human Selenoprotein N, SELN ELISA Kit

Human Selenoprotein N, SELN ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 1.53ng/ml

Detection range: 3-900ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 1.53ng/ml

Detection range: 3-900ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Selenoprotein N

Code

E4240hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

1.53ng/ml

Detection range

3-900ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

[Isoform 2]: Plays an important role in cell protection against oxidative stress and in the regulation of redox-related calcium homeostasis. Regulates the calcium level of the ER by protecting the calcium pump ATP2A2 against the oxidoreductase ERO1A-mediated oxidative damage. Within the ER, ERO1A activity increases the concentration of H(2)O(2), which attacks the luminal thiols in ATP2A2 and thus leads to cysteinyl sulfenic acid formation (-SOH) and SEPN1 reduces the SOH back to free thiol (-SH), thus restoring ATP2A2 activity (PubMed: 25452428). Acts as a modulator of ryanodine receptor (RyR) activity: protects RyR from oxidation due to increased oxidative stress, or directly controls the RyR redox state, regulating the RyR-mediated calcium mobilization required for normal muscle development and differentiation (PubMed: 19557870, PubMed: 18713863). Essential for muscle regeneration and satellite cell maintenance in skeletal muscle (PubMed: 21131290).

UniProt accession

MASS(Da)

65,813

GeneID

57190

Synonyms

SELENON;Selenoprotein N;SelN

Gene names

SELENON

Research area

Others

Target protein

SELENON

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E4240hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
480ng/ml 2.551 2.537 2.489
2.522
240ng/ml 1.563 1.567 1.519
1.57
120ng/ml 0.769 0.75 0.702
0.731
60ng/ml 0.426 0.463 0.416
0.501
30ng/ml 0.215 0.208 0.161
0.202
0ng/ml 0.036 0.048 0
0.059

Precision

We measured random samples of E4240hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 234.9 7.11 3
Intra-Assay 2 18 87.7 4.22 4.8
Intra-Assay 3 18 178.9 9.47 5.3

Protocols

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 48T

    E4240hu

    $320.00

  • 96T

    E4240hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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