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Home > Product > ELISA Kit > Human Ephrin Type-A Receptor 4, EPHA4 ELISA Kit

Human Ephrin Type-A Receptor 4, EPHA4 ELISA Kit

Human Ephrin Type-A Receptor 4, EPHA4 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 6.97ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 6.97ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Ephrin Type-A Receptor 4

Code

E4550Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

6.97ng/L

Detection range

15-3000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Receptor tyrosine kinase which binds membrane-bound ephrin family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Highly promiscuous, it has the unique property among Eph receptors to bind and to be physiologically activated by both GPI-anchored ephrin-A and transmembrane ephrin-B ligands including EFNA1 and EFNB3. Upon activation by ephrin ligands, modulates cell morphology and integrin-dependent cell adhesion through regulation of the Rac, Rap and Rho GTPases activity. Plays an important role in the development of the nervous system controlling different steps of axonal guidance including the establishment of the corticospinal projections. May also control the segregation of motor and sensory axons during neuromuscular circuit development. In addition to its role in axonal guidance plays a role in synaptic plasticity. Activated by EFNA1 phosphorylates CDK5 at 'Tyr-15' which in turn phosphorylates NGEF regulating RHOA and dendritic spine morphogenesis. In the nervous system, plays also a role in repair after injury preventing axonal regeneration and in angiogenesis playing a role in central nervous system vascular formation. Additionally, its promiscuity makes it available to participate in a variety of cell-cell signaling regulating for instance the development of the thymic epithelium. During development of the cochlear organ of Corti, regulates pillar cell separation by forming a ternary complex with ADAM10 and CADH1 which facilitates the cleavage of CADH1 by ADAM10 and disruption of adherens junctions (By similarity).

UniProt accession

MASS(Da)

109,860

GeneID

2043

Synonyms

EK8;EPHA 4;EPHA4;EPH-like kinase 8;Ephrin type-A receptor 4;hEK8;Tyrosine-protein kinase receptor SEK;Tyrosine-protein kinase TYRO1

Gene names

EPHA4

Research area

Signal Transduction

Target protein

EPHA4

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E4550Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1600ng/L 1.813 1.955 1.912
2.097
800ng/L 0.981 0.961 0.918
0.941
400ng/L 0.556 0.567 0.524
0.578
200ng/L 0.206 0.234 0.191
0.262
100ng/L 0.074 0.11 0.067
0.145
0ng/L 0.037 0.043 0
0.049

Precision

We measured random samples of E4550Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 288.8 15.01 5.2
Intra-Assay 2 18 924 44.37 4.8
Intra-Assay 3 18 1162.1 31.97 2.8

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E4550Hu

    $320.00

  • 96T

    E4550Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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