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Home > Product > ELISA Kit > Human Glutamine Synthetase, GS ELISA Kit

Human Glutamine Synthetase, GS ELISA Kit

Human Glutamine Synthetase, GS ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 2.56ng/L

Detection range: 5-1000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 2.56ng/L

Detection range: 5-1000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Glutamine Synthetase

Code

E4569Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

2.56ng/L

Detection range

5-1000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Glutamine synthetase that catalyzes the ATP-dependent conversion of glutamate and ammonia to glutamine (PubMed: 30158707, PubMed: 16267323). Its role depends on tissue localization: in the brain, it regulates the levels of toxic ammonia and converts neurotoxic glutamate to harmless glutamine, whereas in the liver, it is one of the enzymes responsible for the removal of ammonia (By similarity). Essential for proliferation of fetal skin fibroblasts (PubMed: 18662667). Independently of its glutamine synthetase activity, required for endothelial cell migration during vascular development: acts by regulating membrane localization and activation of the GTPase RHOJ, possibly by promoting RHOJ palmitoylation (PubMed: 30158707). May act as a palmitoyltransferase for RHOJ: able to autopalmitoylate and then transfer the palmitoyl group to RHOJ (PubMed: 30158707). Plays a role in ribosomal 40S subunit biogenesis (PubMed: 26711351).

UniProt accession

MASS(Da)

42,064

GeneID

2752

Synonyms

GLUL;Glutamate--ammonia ligase;Glutamine synthetase;GS;Palmitoyltransferase GLUL

Gene names

GLUL

Research area

Neuroscience

Target protein

GLUL

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E4569Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
640ng/L 2.271 2.274 2.24
2.276
320ng/L 1.281 1.272 1.238
1.264
160ng/L 0.563 0.607 0.573
0.651
80ng/L 0.298 0.306 0.272
0.315
40ng/L 0.164 0.155 0.121
0.146
0ng/L 0.016 0.034 0
0.052

Precision

We measured random samples of E4569Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 473.3 13.29 2.8
Intra-Assay 2 18 258.2 11.2 4.3
Intra-Assay 3 18 116.1 4.85 4.2

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E4569Hu

    $320.00

  • 96T

    E4569Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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