Human Actin-related Protein 3, ACTR3 ELISA Kit

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Home > Product > ELISA Kit > Human Actin-related Protein 3, ACTR3 ELISA Kit

Human Actin-related Protein 3, ACTR3 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 12.05ng/L

Detection range: 20-4000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 12.05ng/L

Detection range: 20-4000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Actin-Related Protein 3

Code

E4623Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

12.05ng/L

Detection range

20-4000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

ATP-binding component of the Arp2/3 complex, a multiprotein complex that mediates actin polymerization upon stimulation by nucleation-promoting factor (NPF) (PubMed: 9000076). The Arp2/3 complex mediates the formation of branched actin networks in the cytoplasm, providing the force for cell motility (PubMed: 9000076). Seems to contact the pointed end of the daughter actin filament (PubMed: 9000076). In podocytes, required for the formation of lamellipodia downstream of AVIL and PLCE1 regulation (PubMed: 29058690). In addition to its role in the cytoplasmic cytoskeleton, the Arp2/3 complex also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA (PubMed: 17220302, PubMed: 29925947). The Arp2/3 complex promotes homologous recombination (HR) repair in response to DNA damage by promoting nuclear actin polymerization, leading to drive motility of double-strand breaks (DSBs) (PubMed: 29925947). Plays a role in ciliogenesis (PubMed: 20393563).

UniProt accession

P61158

MASS（Da）

47,371

GeneID

10096

Synonyms

Actin-like protein 3;Actin-related protein 3;ACTR 3;ACTR3

Gene names

ACTR3

Research area

Others

Target protein

ACTR3

Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Protocol data

These standard curves of E4623Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2400ng/L	2.088	2.237	2.189
	2.387
1200ng/L	1.342	1.369	1.321
	1.397
600ng/L	0.579	0.658	0.61
	0.736
300ng/L	0.439	0.41	0.362
	0.38
150ng/L	0.19	0.193	0.145
	0.195
0ng/L	0.054	0.048	0
	0.042

Precision

We measured random samples of E4623Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	1404.1	41.8	3
Intra-Assay	2	18	1061.2	44.8	4.2
Intra-Assay	3	18	483.9	22.57	4.7

Protocols

> Protocol Booklet

References

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Associated products

Datasheet

Size

Code

Price

48T

E4623Hu

$320.00
96T

E4623Hu

$458.00

Add to Cart

Lead time: Within two weeks

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