Human Hypoxia-inducible Factor 3 Alpha, HIF-3A ELISA Kit

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Home > Product > ELISA Kit > Human Hypoxia-inducible Factor 3 Alpha, HIF-3A ELISA Kit

Human Hypoxia-inducible Factor 3 Alpha, HIF-3A ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 3.32ng/L

Detection range: 6-1500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 3.32ng/L

Detection range: 6-1500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Hypoxia-Inducible Factor 3 Alpha

Code

E4763Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

3.32ng/L

Detection range

6-1500ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Acts as a transcriptional regulator in adaptive response to low oxygen tension. Acts as a regulator of hypoxia-inducible gene expression (PubMed: 11573933, PubMed: 16126907, PubMed: 19694616, PubMed: 20416395, PubMed: 21069422). Functions as an inhibitor of angiogenesis in hypoxic cells of the cornea. Plays a role in the development of the cardiorespiratory system. May also be involved in apoptosis (By similarity). [Isoform 2]: Attenuates the ability of transcription factor HIF1A to bind to hypoxia-responsive elements (HRE) located within the enhancer/promoter of hypoxia-inducible target genes and hence inhibits HRE-driven transcriptional activation. Also inhibits hypoxia-inducible ARNT-mediated gene expression. [Isoform 3]: Attenuates the ability of transcription factor HIF1A to bind to hypoxia-responsive elements (HRE) located within the enhancer/promoter of hypoxia-inducible target genes and hence inhibits HRE-driven transcriptional activation. [Isoform 4]: Attenuates the ability of transcription factor HIF1A and EPAS1/HIF2A to bind to hypoxia-responsive elements (HRE) located within the enhancer/promoter of hypoxia-inducible target genes and hence inhibits HRE-driven transcriptional activation (PubMed: 16126907, PubMed: 17998805, PubMed: 19694616, PubMed: 20416395). May act as a tumor suppressor and inhibits malignant cell transformation (PubMed: 17998805). [Isoform 5]: Attenuates the ability of transcription factor HIF1A to bind to hypoxia-responsive elements (HRE) located within the enhancer/promoter of hypoxia-inducible target genes and hence inhibits HRE-driven transcriptional activation.

UniProt accession

Q9Y2N7

MASS（Da）

72,433

GeneID

64344

Synonyms

Basic-helix-loop-helix-PAS protein MOP7;bHLHe17;Class E basic helix-loop-helix protein 17;HIF3A;HIF3-alpha;HIF-3-alpha;HIF3-alpha-1;Hypoxia-inducible factor 3-alpha;Inhibitory PAS domain protein;IPAS;Member of PAS protein 7;PAS domain-containing protein 7

Gene names

HIF3A

Research area

Others

Target protein

HIF3A

Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Protocol data

These standard curves of E4763Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
800ng/L	1.865	1.939	1.894
	2.013
400ng/L	0.875	1.003	0.958
	1.13
200ng/L	0.605	0.613	0.568
	0.62
100ng/L	0.283	0.273	0.229
	0.263
50ng/L	0.14	0.107	0.063
	0.075
0ng/L	0.044	0.044	0
	0.045

Precision

We measured random samples of E4763Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	289.3	12.55	4.3
Intra-Assay	2	18	754.7	25.28	3.3
Intra-Assay	3	18	126.5	5.42	4.3

Protocols

> Protocol Booklet

References

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Associated products

Datasheet

Size

Code

Price

48T

E4763Hu

$320.00
96T

E4763Hu

$458.00

Add to Cart

Lead time: Within two weeks

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