Human Microtubule-associated Protein 1B, MAP1B ELISA Kit

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Home > Product > ELISA Kit > Human Microtubule-associated Protein 1B, MAP1B ELISA Kit

Human Microtubule-associated Protein 1B, MAP1B ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 7.58ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 7.58ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Microtubule-Associated Protein 1B

Code

E4782Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

7.58ng/L

Detection range

15-3000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Facilitates tyrosination of alpha-tubulin in neuronal microtubules (By similarity). Phosphorylated MAP1B may play a role in the cytoskeletal changes that accompany neurite extension. Possibly MAP1B binds to at least two tubulin subunits in the polymer, and this bridging of subunits might be involved in nucleating microtubule polymerization and in stabilizing microtubules. Acts as a positive cofactor in DAPK1-mediated autophagic vesicle formation and membrane blebbing.

UniProt accession

P46821

MASS（Da）

270,634

GeneID

4131

Synonyms

MAP1 light chain LC1;MAP1B;MAP-1B;MAP1B heavy chain;Microtubule-associated protein 1B

Gene names

MAP1B

Research area

Neuroscience

Target protein

MAP1B

Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Protocol data

These standard curves of E4782Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1600ng/L	2.122	2.162	2.129
	2.201
800ng/L	1.41	1.384	1.351
	1.357
400ng/L	0.653	0.642	0.609
	0.63
200ng/L	0.39	0.354	0.321
	0.317
100ng/L	0.201	0.185	0.153
	0.17
0ng/L	0.023	0.032	0
	0.042

Precision

We measured random samples of E4782Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	388.2	18.02	4.6
Intra-Assay	2	18	1102.6	39.46	3.6
Intra-Assay	3	18	476.4	19.25	4

Protocols

> Protocol Booklet

References

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Associated products

Datasheet

Size

Code

Price

48T

E4782Hu

$320.00
96T

E4782Hu

$458.00

Add to Cart

Lead time: Within two weeks

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