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Home > Product > ELISA Kit > Human Alpha-2-Macroglobulin-like Protein 1, A2ML1 ELISA Kit

Human Alpha-2-Macroglobulin-like Protein 1, A2ML1 ELISA Kit

Human Alpha-2-Macroglobulin-like Protein 1, A2ML1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 2.29ng/mL

Detection range: 5-1000ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 2.29ng/mL

Detection range: 5-1000ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Alpha-2-Macroglobulin-Like Protein 1

Code

E4926Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

2.29ng/mL

Detection range

5-1000ng/mL

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a Peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase (By similarity). Displays inhibitory activity against chymotrypsin, papain, thermolysin, subtilisin A and, to a lesser extent, elastase but not trypsin. May play an important role during desquamation by inhibiting extracellular proteases.

UniProt accession

MASS(Da)

161,107

GeneID

144568

Synonyms

A2ML1;Alpha-2-macroglobulin-like protein 1;C3 and PZP-like alpha-2-macroglobulin domain-containing protein 9

Gene names

A2ML1

Research area

Others

Target protein

A2ML1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E4926Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
640ng/mL 2.401 2.39 2.365
2.38
320ng/mL 1.23 1.214 1.188
1.197
160ng/mL 0.601 0.583 0.558
0.565
80ng/mL 0.396 0.388 0.363
0.38
40ng/mL 0.152 0.135 0.11
0.118
0ng/mL 0.018 0.025 0
0.033

Precision

We measured random samples of E4926Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 299.7 13.62 4.5
Intra-Assay 2 18 431.5 11.66 2.7
Intra-Assay 3 18 117.7 6.68 5.7

Protocols

References

Customer reviews and Q&As

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Associated products

Product size

Price

  • 48T

    $320.00

  • 96T

    $458.00

Add to Cart

Lead time: Within one week

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