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Home > Product > ELISA Kit > Human Alpha-Enolase, ENO1 ELISA Kit

Human Alpha-Enolase, ENO1 ELISA Kit

Human Alpha-Enolase, ENO1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.048ng/ml

Detection range: 0.1-40ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.048ng/ml

Detection range: 0.1-40ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Alpha-Enolase

Code

E4933Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.048ng/ml

Detection range

0.1-40ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Glycolytic enzyme the catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate (PubMed: 29775581, PubMed: 1369209). In addition to glycolysis, involved in various processes such as growth control, hypoxia tolerance and allergic responses (PubMed: 2005901, PubMed: 10802057, PubMed: 12666133, PubMed: 29775581). May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons (PubMed: 12666133). Stimulates immunoglobulin production (PubMed: 1369209). MBP1 binds to the myc promoter and acts as a transcriptional repressor. May be a tumor suppressor.

UniProt accession

MASS(Da)

47,169

GeneID

2023

Synonyms

2-phospho-D-glycerate hydro-lyase;Alpha-enolase;C-myc promoter-binding protein;ENO 1;ENO1;Enolase 1;MBP-1;MPB-1;NNE;Non-neural enolase;Phosphopyruvate hydratase;Plasminogen-binding protein

Gene names

ENO1

Research area

Metabolism

Target protein

ENO1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E4933Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
24ng/ml 2.348 2.343 2.281
2.339
12ng/ml 1.426 1.385 1.322
1.344
6ng/ml 0.731 0.754 0.691
0.776
3ng/ml 0.44 0.446 0.383
0.452
1.5ng/ml 0.268 0.296 0.233
0.324
0ng/ml 0.056 0.063 0
0.07

Precision

We measured random samples of E4933Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 6.5 0.34 5.3
Intra-Assay 2 18 16.6 0.5 3
Intra-Assay 3 18 3.9 0.23 5.9

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E4933Hu

    $320.00

  • 96T

    E4933Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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