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Home > Product > ELISA Kit > Human Caspase Recruitment Domain-containing Protein 9, CARD9 ELISA Kit

Human Caspase Recruitment Domain-containing Protein 9, CARD9 ELISA Kit

Human Caspase Recruitment Domain-containing Protein 9, CARD9 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 5.03ng/L

Detection range: 10-2000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 5.03ng/L

Detection range: 10-2000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Caspase Recruitment Domain-Containing Protein 9

Code

E4992Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

5.03ng/L

Detection range

10-2000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Adapter protein that plays a key role in innate immune response to a number of intracellular pathogens, such as C.albicans and L.monocytogenes. Is at the crossroads of ITAM-tyrosine kinase and the Toll-like receptors (TLR) and NOD2 signaling pathways. Probably controls various innate immune response pathways depending on the intracellular pathogen. In response to L.monocytogenes infection, acts by connecting NOD2 recognition of peptidoglycan to downstream activation of MAP kinases (MAPK) without activating NF-kappa-B. Also involved in activation of myeloid cells via classical ITAM-associated receptors and TLR: required for TLR-mediated activation of MAPK, while it is not required for TLR-induced activation of NF-kappa-B (By similarity). Controls CLEC7A (dectin-1)-mediated myeloid cell activation induced by the yeast cell wall component zymosan, leading to cytokine production and innate anti-fungal immunity: acts by regulating BCL10-MALT1-mediated NF-kappa-B activation pathway. Activates NF-kappa-B via BCL10. In response to the hyphal form of C.albicans, mediates CLEC6A (dectin-2)-induced I-kappa-B kinase ubiquitination, leading to NF-kappa-B activation via interaction with BCL10. In response to fungal infection, may be required for the development and subsequent differentiation of interleukin 17-producing T helper (TH-17) cells.

UniProt accession

MASS(Da)

62,241

GeneID

64170

Synonyms

CARD 9;CARD9;Caspase recruitment domain-containing protein 9;hCARD9

Gene names

CARD9

Research area

Others

Target protein

CARD9

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E4992Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1200ng/L 2.148 2.154 2.108
2.159
600ng/L 1.34 1.435 1.39
1.53
300ng/L 0.931 0.908 0.863
0.886
150ng/L 0.553 0.547 0.501
0.54
75ng/L 0.275 0.291 0.246
0.308
0ng/L 0.053 0.046 0
0.038

Precision

We measured random samples of E4992Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 659.4 28.96 4.4
Intra-Assay 2 18 244 13.28 5.4
Intra-Assay 3 18 133.9 9.16 6.8

Protocols

References

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 48T

    E4992Hu

    $320.00

  • 96T

    E4992Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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