Human Clip-Associating Protein 2, CLASP2 ELISA Kit

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Home > Product > ELISA Kit > Human Clip-Associating Protein 2, CLASP2 ELISA Kit

Human Clip-Associating Protein 2, CLASP2 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 7.12ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 7.12ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Clip-Associating Protein 2

Code

E5053Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

7.12ng/L

Detection range

15-3000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Microtubule plus-end tracking protein that promotes the stabilization of dynamic microtubules (PubMed: 26003921). Involved in the nucleation of noncentrosomal microtubules originating from the trans-Golgi network (TGN). Required for the polarization of the cytoplasmic microtubule arrays in migrating cells towards the leading edge of the cell. May act at the cell cortex to enhance the frequency of rescue of depolymerizing microtubules by attaching their plus-ends to cortical platforms composed of ERC1 and PHLDB2 (PubMed: 16824950). This cortical microtubule stabilizing activity is regulated at least in part by phosphatidylinositol 3-kinase signaling. Also performs a similar stabilizing function at the kinetochore which is essential for the bipolar alignment of chromosomes on the mitotic spindle (PubMed: 16866869, PubMed: 16914514). Acts as a mediator of ERBB2-dependent stabilization of microtubules at the cell cortex.

UniProt accession

O75122

MASS（Da）

141,064

GeneID

23122

Synonyms

CLASP 2;CLASP2;CLIP-associating protein 2;Cytoplasmic linker-associated protein 2;hOrbit2;Protein Orbit homolog 2

Gene names

CLASP2

Research area

Cell Biology

Target protein

CLASP2

Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Protocol data

These standard curves of E5053Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1600ng/L	2.258	2.205	2.169
	2.152
800ng/L	1.527	1.545	1.509
	1.563
400ng/L	0.747	0.76	0.724
	0.774
200ng/L	0.406	0.368	0.332
	0.33
100ng/L	0.156	0.157	0.121
	0.159
0ng/L	0.034	0.037	0
	0.04

Precision

We measured random samples of E5053Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	313.1	13.88	4.4
Intra-Assay	2	18	831.5	25.35	3
Intra-Assay	3	18	391.3	12.64	3.2

Protocols

> Protocol Booklet

References

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Associated products

Datasheet

Size

Code

Price

48T

E5053Hu

$320.00
96T

E5053Hu

$458.00

Add to Cart

Lead time: Within two weeks

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