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Home > Product > ELISA Kit > Human Cytochrome P450 26A1, CYP26A1 ELISA Kit

Human Cytochrome P450 26A1, CYP26A1 ELISA Kit

Human Cytochrome P450 26A1, CYP26A1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 4.9ng/L

Detection range: 10-2000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 4.9ng/L

Detection range: 10-2000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Cytochrome P450 26A1

Code

E5159Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

4.9ng/L

Detection range

10-2000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

A cytochrome P450 monooxygenase involved in the metabolism of all-trans retinoic acid (atRA), a signaling molecule that binds to retinoic acid receptors and regulates gene transcription. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase). Catalyzes the hydroxylation of carbon hydrogen bonds of atRA primarily at C-4 and C-18. Has no activity toward 9-cis and 13-cis retinoic acid stereoisomers (PubMed: 22020119, PubMed: 9228017, PubMed: 9716180). May play a role in the oxidative metabolism of xenobiotics such as tazarotenic acid (PubMed: 26937021).

UniProt accession

MASS(Da)

56,199

GeneID

1592

Synonyms

CYP26A1;Cytochrome P450 26A1;Cytochrome P450 retinoic acid-inactivating 1;Cytochrome P450RAI;hP450RAI;Retinoic acid 4-hydroxylase;Retinoic acid-metabolizing cytochrome

Gene names

CYP26A1

Research area

Others

Target protein

CYP26A1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E5159Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1200ng/L 2.206 2.284 2.248
2.362
600ng/L 1.403 1.372 1.335
1.34
300ng/L 0.788 0.697 0.661
0.606
150ng/L 0.379 0.382 0.346
0.385
75ng/L 0.214 0.185 0.149
0.156
0ng/L 0.038 0.036 0
0.035

Precision

We measured random samples of E5159Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 503.1 25.88 5.1
Intra-Assay 2 18 718.4 21.43 3
Intra-Assay 3 18 196.2 8.61 4.4

Protocols

References

Customer reviews and Q&As

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Associated products

Product size

Price

  • 48T

    $320.00

  • 96T

    $458.00

Add to Cart

Lead time: Within one week

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