Tel:86 021 3100 7137

Share with us your publications using BT LAB product and get free products.
Home > Product > ELISA Kit > Human Dedicator of Cytokinesis Protein 1, DOCK1 ELISA Kit

Human Dedicator of Cytokinesis Protein 1, DOCK1 ELISA Kit

Human Dedicator of Cytokinesis Protein 1, DOCK1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 9.72ng/L

Detection range: 20-6000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 9.72ng/L

Detection range: 20-6000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Dedicator of Cytokinesis Protein 1

Code

E5173Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

9.72ng/L

Detection range

20-6000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Along with DOCK1, mediates CRK/CRKL regulation of epithelial and endothelial cell spreading and migration on type IV collagen (PubMed: 19004829). Functions as a guanine nucleotide exchange factor (GEF), which activates Rac Rho small GTPases by exchanging bound GDP for free GTP. Its GEF activity may be enhanced by ELMO1 (PubMed: 8657152).

UniProt accession

MASS(Da)

215,346

GeneID

1793

Synonyms

180 kDa protein downstream of CRK;Dedicator of cytokinesis protein 1;DOCK 1;DOCK1;DOCK180

Gene names

DOCK1

Research area

Others

Target protein

DOCK1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E5173Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
3200ng/L 2.157 2.139 2.098
2.122
1600ng/L 1.55 1.586 1.544
1.622
800ng/L 0.966 0.966 0.925
0.967
400ng/L 0.779 0.735 0.694
0.692
200ng/L 0.428 0.445 0.404
0.462
0ng/L 0.031 0.041 0
0.052

Precision

We measured random samples of E5173Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 254.4 18.51 7.3
Intra-Assay 2 18 560.6 35.49 6.3
Intra-Assay 3 18 1095 54.14 4.9

Protocols

References

Customer reviews and Q&As

No reviews
No reviews

Product size

Price

  • 48T

    $320.00

  • 96T

    $458.00

Add to Cart

Lead time: Within one week

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

Newsletter

Sign up

Newsletter

Sign up