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Home > Product > ELISA Kit > Human Deoxyribonuclease Gamma, DNASE1L3 ELISA Kit

Human Deoxyribonuclease Gamma, DNASE1L3 ELISA Kit

Human Deoxyribonuclease Gamma, DNASE1L3 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 7.55ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 7.55ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Deoxyribonuclease Gamma

Code

E5176Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

7.55ng/L

Detection range

15-3000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Has DNA hydrolytic activity. Is capable of both single- and double-stranded DNA cleavage, producing DNA fragments with 3'-OH ends (By similarity). Can cleave chromatin to nucleosomal units and cleaves nucleosomal and liposome-coated DNA (PubMed: 9070308, PubMed: 9714828, PubMed: 14646506, PubMed: 10807908, PubMed: 27293190). Acts in internucleosomal DNA fragmentation (INDF) during apoptosis and necrosis (PubMed: 23229555, PubMed: 24312463). The role in apoptosis includes myogenic and neuronal differentiation, and BCR-mediated clonal deletion of self-reactive B cells (By similarity). Is active on chromatin in apoptotic cell-derived membrane-coated microparticles and thus suppresses anti-DNA autoimmunity (PubMed: 27293190). Together with DNASE1, plays a key role in degrading neutrophil extracellular traps (NETs) (By similarity). NETs are mainly composed of DNA fibers and are released by neutrophils to bind pathogens during inflammation (By similarity). Degradation of intravascular NETs by DNASE1 and DNASE1L3 is required to prevent formation of clots that obstruct blood vessels and cause organ damage following inflammation (By similarity).

UniProt accession

MASS(Da)

35,504

GeneID

1776

Synonyms

Deoxyribonuclease gamma;Deoxyribonuclease I-like 3;DNASE 1L3;DNase gamma;DNase I homolog protein DHP2;DNase I-like 3;DNASE1L3;Liver and spleen DNase;LSD;LS-DNAse

Gene names

DNASE1L3

Research area

Epigenetics and Nuclear Signaling

Target protein

DNASE1L3

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E5176Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1600ng/L 2.34 2.344 2.307
2.348
800ng/L 1.517 1.481 1.444
1.444
400ng/L 0.94 0.921 0.884
0.902
200ng/L 0.5 0.476 0.438
0.451
100ng/L 0.303 0.263 0.226
0.223
0ng/L 0.039 0.037 0
0.035

Precision

We measured random samples of E5176Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 128.6 7.66 6
Intra-Assay 2 18 576.6 34.6 6
Intra-Assay 3 18 720.6 28.69 4

Protocols

References

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Associated products

Product size

Price

  • 48T

    $320.00

  • 96T

    $458.00

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Lead time: Within one week

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