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Home > Product > ELISA Kit > Human Ephrin Type-A Receptor 1, EPHA1 ELISA Kit

Human Ephrin Type-A Receptor 1, EPHA1 ELISA Kit

Human Ephrin Type-A Receptor 1, EPHA1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.031ng/mL

Detection range: 0.05-20ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.031ng/mL

Detection range: 0.05-20ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Ephrin Type-A Receptor 1

Code

E5244Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.031ng/mL

Detection range

0.05-20ng/mL

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Receptor tyrosine kinase which binds promiscuously membrane-bound ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Binds with a low affinity EFNA3 and EFNA4 and with a high affinity to EFNA1 which most probably constitutes its cognate/functional ligand. Upon activation by EFNA1 induces cell attachment to the extracellular matrix inhibiting cell spreading and motility through regulation of ILK and downstream RHOA and RAC. Plays also a role in angiogenesis and regulates cell proliferation. May play a role in apoptosis.

UniProt accession

MASS(Da)

108,127

GeneID

2041

Synonyms

EPH tyrosine kinase;EPH tyrosine kinase 1;EPHA 1;EPHA1;Ephrin type-A receptor 1;Erythropoietin-producing hepatoma receptor;hEpha1;Tyrosine-protein kinase receptor EPH

Gene names

EPHA1

Research area

Others

Target protein

EPHA1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E5244Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
12.8ng/mL 2.401 2.52 2.474
2.64
6.4ng/mL 1.397 1.367 1.321
1.338
3.2ng/mL 0.823 0.841 0.794
0.859
1.6ng/mL 0.429 0.419 0.373
0.41
0.8ng/mL 0.282 0.257 0.211
0.232
0ng/mL 0.04 0.046 0
0.052

Precision

We measured random samples of E5244Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 5.1 0.26 5
Intra-Assay 2 18 7.6 0.18 2.4
Intra-Assay 3 18 1.2 0.08 7

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E5244Hu

    $320.00

  • 96T

    E5244Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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