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Home > Product > ELISA Kit > Human Ephrin Type-A Receptor 2, EPHA2 ELISA Kit

Human Ephrin Type-A Receptor 2, EPHA2 ELISA Kit

Human Ephrin Type-A Receptor 2, EPHA2 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.026ng/ml

Detection range: 0.05-20ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.026ng/ml

Detection range: 0.05-20ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Ephrin Type-A Receptor 2

Code

E5246Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.026ng/ml

Detection range

0.05-20ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Receptor tyrosine kinase which binds promiscuously membrane-bound ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Activated by the ligand ephrin-A1/EFNA1 regulates migration, integrin-mediated adhesion, proliferation and differentiation of cells. Regulates cell adhesion and differentiation through DSG1/desmoglein-1 and inhibition of the ERK1/ERK2 (MAPK3/MAPK1, respectively) signaling pathway. May also participate in UV radiation-induced apoptosis and have a ligand-independent stimulatory effect on chemotactic cell migration. During development, may function in distinctive aspects of pattern formation and subsequently in development of several fetal tissues. Involved for instance in angiogenesis, in early hindbrain development and epithelial proliferation and branching morphogenesis during mammary gland development. Engaged by the ligand ephrin-A5/EFNA5 may regulate lens fiber cells shape and interactions and be important for lens transparency development and maintenance. With ephrin-A2/EFNA2 may play a role in bone remodeling through regulation of osteoclastogenesis and osteoblastogenesis. (Microbial infection) Acts as a receptor for hepatitis C virus (HCV) in hepatocytes and facilitates its cell entry. Mediates HCV entry by promoting the formation of the CD81-CLDN1 receptor complexes that are essential for HCV entry and by enhancing membrane fusion of cells expressing HCV envelope glycoproteins.

UniProt accession

MASS(Da)

108,266

GeneID

1969

Synonyms

EPHA 2;EPHA2;Ephrin type-A receptor 2;Epithelial cell kinase;Tyrosine-protein kinase receptor ECK

Gene names

EPHA2

Research area

Cardiovascular

Target protein

EPHA2

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E5246Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
12ng/ml 1.97 2.059 2.02
2.147
6ng/ml 1.498 1.443 1.405
1.389
3ng/ml 0.639 0.638 0.599
0.636
1.5ng/ml 0.364 0.359 0.321
0.354
0.75ng/ml 0.162 0.175 0.137
0.188
0ng/ml 0.044 0.038 0
0.033

Precision

We measured random samples of E5246Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 3.4 0.14 4.1
Intra-Assay 2 18 7.4 0.28 3.7
Intra-Assay 3 18 2.4 0.09 3.9

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E5246Hu

    $320.00

  • 96T

    E5246Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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