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Home > Product > ELISA Kit > Human Erlin-2, ERLIN2 ELISA Kit

Human Erlin-2, ERLIN2 ELISA Kit

Human Erlin-2, ERLIN2 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 5.15ng/L

Detection range: 10-2000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 5.15ng/L

Detection range: 10-2000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Erlin-2

Code

E5260Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

5.15ng/L

Detection range

10-2000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Component of the ERLIN1/ERLIN2 complex which mediates the endoplasmic reticulum-associated degradation (ERAD) of inositol 1,4,5-trisphosphate receptors (IP3Rs) such as ITPR1 (PubMed: 19240031, PubMed: 17502376). Promotes sterol-accelerated ERAD of HMGCR probably implicating an AMFR/gp78-containing ubiquitin ligase complex (PubMed: 21343306). Involved in regulation of cellular cholesterol homeostasis by regulation the SREBP signaling pathway. May promote ER retention of the SCAP-SREBF complex (PubMed: 24217618).

UniProt accession

MASS(Da)

37,840

GeneID

11160

Synonyms

Endoplasmic reticulum lipid raft-associated protein 2;ERLIN 2;ERLIN2;Erlin-2;SPFH domain-containing protein 2;Stomatin-prohibitin-flotillin-HflC/K domain-containing protein 2

Gene names

ERLIN2

Research area

Neuroscience

Target protein

ERLIN2

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E5260Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1280ng/L 2.26 2.244 2.183
2.229
640ng/L 1.322 1.376 1.315
1.431
320ng/L 0.719 0.793 0.732
0.868
160ng/L 0.496 0.511 0.45
0.526
80ng/L 0.26 0.282 0.221
0.304
0ng/L 0.062 0.061 0
0.06

Precision

We measured random samples of E5260Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 953 32.87 3.4
Intra-Assay 2 18 274.2 20.47 7.5
Intra-Assay 3 18 513.4 31.55 6.1

Protocols

References

Customer reviews and Q&As

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Associated products

Product size

Price

  • 48T

    $320.00

  • 96T

    $458.00

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Lead time: Within one week

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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