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Home > Product > ELISA Kit > Human Fatty-acid Amidehydrolase 1, FAAH ELISA Kit

Human Fatty-acid Amidehydrolase 1, FAAH ELISA Kit

Human Fatty-acid Amidehydrolase 1, FAAH ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 9.46ng/L

Detection range: 20-4500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 9.46ng/L

Detection range: 20-4500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Fatty-Acid Amide Hydrolase 1

Code

E5280Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

9.46ng/L

Detection range

20-4500ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Catalyzes the hydrolysis of endogenous amidated lipids like the sleep-inducing lipid oleamide ((9Z)-octadecenamide), the endocannabinoid anandamide (N-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-ethanolamine), as well as other fatty amides, to their corresponding fatty acids, thereby regulating the signaling functions of these molecules (PubMed: 9122178, PubMed: 17015445, PubMed: 19926788). Hydrolyzes polyunsaturated substrate anandamide preferentially as compared to monounsaturated substrates (PubMed: 9122178, PubMed: 17015445). It can also catalyze the hydrolysis of the endocannabinoid 2-arachidonoylglycerol (2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-glycerol) (PubMed: 21049984). FAAH cooperates with PM20D1 in the hydrolysis of amino acid-conjugated fatty acids such as N-fatty acyl glycine and N-fatty acyl-L-serine, thereby acting as a physiological regulator of specific subsets of intracellular, but not of extracellular, N-fatty acyl amino acids (By similarity).

UniProt accession

MASS(Da)

63,066

GeneID

2166

Synonyms

Anandamide amidohydrolase 1;FAAH;Fatty acid ester hydrolase;Fatty-acid amide hydrolase 1;Oleamide hydrolase 1

Gene names

FAAH

Research area

Metabolism

Target protein

FAAH

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E5280Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
2400ng/L 2.194 2.158 2.105
2.122
1200ng/L 1.347 1.429 1.377
1.511
600ng/L 0.782 0.79 0.738
0.798
300ng/L 0.538 0.522 0.47
0.506
150ng/L 0.285 0.25 0.198
0.216
0ng/L 0.068 0.052 0
0.036

Precision

We measured random samples of E5280Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 1078 34.28 3.2
Intra-Assay 2 18 645.6 32.37 5
Intra-Assay 3 18 267 13.21 4.9

Protocols

References

Customer reviews and Q&As

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Product size

Price

  • 48T

    $320.00

  • 96T

    $458.00

Add to Cart

Lead time: Within one week

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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