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Home > Product > ELISA Kit > Human Heat Shock Protein Hsp 90-beta, HSP90AB1 ELISA Kit

Human Heat Shock Protein Hsp 90-beta, HSP90AB1 ELISA Kit

Human Heat Shock Protein Hsp 90-beta, HSP90AB1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.36ng/mL

Detection range: 0.5-200ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.36ng/mL

Detection range: 0.5-200ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Heat Shock Protein Hsp 90-Beta

Code

E5382Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.36ng/mL

Detection range

0.5-200ng/mL

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function (PubMed: 16478993, PubMed: 19696785). Engages with a range of client protein classes via its interaction with various co-chaperone proteins or complexes, that act as adapters, simultaneously able to interact with the specific client and the central chaperone itself. Recruitment of ATP and co-chaperone followed by client protein forms a functional chaperone. After the completion of the chaperoning process, properly folded client protein and co-chaperone leave HSP90 in an ADP-bound partially open conformation and finally, ADP is released from HSP90 which acquires an open conformation for the next cycle (PubMed: 27295069, PubMed: 26991466). Apart from its chaperone activity, it also plays a role in the regulation of the transcription machinery. HSP90 and its co-chaperones modulate transcription at least at three different levels. They first alter the steady-state levels of certain transcription factors in response to various physiological cues. Second, they modulate the activity of certain epigenetic modifiers, such as histone deacetylases or DNA methyl transferases, and thereby respond to the change in the environment. Third, they participate in the eviction of histones from the promoter region of certain genes and thereby turn on gene expression (PubMed: 25973397). Antagonizes STUB1-mediated inhibition of TGF-beta signaling via inhibition of STUB1-mediated SMAD3 ubiquitination and degradation (PubMed: 24613385). Promotes cell differentiation by chaperoning BIRC2 and thereby protecting from auto-ubiquitination and degradation by the proteasomal machinery (PubMed: 18239673). Main chaperone involved in the phosphorylation/activation of the STAT1 by chaperoning both JAK2 and PRKCE under heat shock and in turn, activates its own transcription (PubMed: 20353823). Involved in the translocation into ERGIC (endoplasmic reticulum-Golgi intermediate compartment) of leaderless cargos (lacking the secretion signal sequence) such as the interleukin 1/IL-1; the translocation process is mediated by the cargo receptor TMED10 (PubMed: 32272059).

UniProt accession

MASS(Da)

83,264

GeneID

3326

Synonyms

Heat shock 84 kDa;Heat shock protein HSP 90-beta;HSP 84;HSP 90;HSP84;HSP90AB1

Gene names

HSP90AB1

Research area

Neuroscience

Target protein

HSP90AB1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E5382Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
128ng/mL 1.99 1.997 1.943
2.004
64ng/mL 1.061 0.989 0.934
0.916
32ng/mL 0.503 0.541 0.486
0.579
16ng/mL 0.162 0.152 0.098
0.142
8ng/mL 0.073 0.064 0.009
0.055
0ng/mL 0.059 0.055 0
0.051

Precision

We measured random samples of E5382Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 110.1 3.23 2.9
Intra-Assay 2 18 27.8 1.02 3.7
Intra-Assay 3 18 49.3 1.85 3.8

Protocols

References

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  • Size

    Code

    Price

  • 48T

    E5382Hu

    $320.00

  • 96T

    E5382Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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