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Home > Product > ELISA Kit > Human Heparan Sulfate Glucosamine 3-O-sulfotransferase 2, HS3ST2 ELISA Kit

Human Heparan Sulfate Glucosamine 3-O-sulfotransferase 2, HS3ST2 ELISA Kit

Human Heparan Sulfate Glucosamine 3-O-sulfotransferase 2, HS3ST2 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 6.97ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 6.97ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Heparan Sulfate Glucosamine 3-O-Sulfotransferase 2

Code

E5386Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

6.97ng/L

Detection range

15-3000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) to catalyze the transfer of a sulfo group to an N-unsubstituted glucosamine linked to a 2-O-sulfo iduronic acid unit on heparan sulfate. Catalyzes the O-sulfation of glucosamine in GlcA2S-GlcNS. Unlike 3-OST-1, does not convert non-anticoagulant heparan sulfate to anticoagulant heparan sulfate.

UniProt accession

MASS(Da)

41,501

GeneID

9956

Synonyms

3-OST-2;h3-OST-2;Heparan sulfate 3-O-sulfotransferase 2;Heparan sulfate D-glucosaminyl 3-O-sulfotransferase 2;Heparan sulfate glucosamine 3-O-sulfotransferase 2;HS3ST2

Gene names

HS3ST2

Research area

Others

Target protein

HS3ST2

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E5386Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1600ng/L 2.224 2.251 2.203
2.277
800ng/L 1.204 1.301 1.254
1.399
400ng/L 0.677 0.68 0.633
0.683
200ng/L 0.466 0.478 0.431
0.49
100ng/L 0.299 0.307 0.26
0.315
0ng/L 0.047 0.047 0
0.047

Precision

We measured random samples of E5386Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 1038.8 36.54 3.5
Intra-Assay 2 18 565.6 31.41 5.6
Intra-Assay 3 18 353.3 21.04 6

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E5386Hu

    $320.00

  • 96T

    E5386Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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