Human Integrin Alpha-5, ITGA5 ELISA Kit

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Home > Product > ELISA Kit > Human Integrin Alpha-5, ITGA5 ELISA Kit

Human Integrin Alpha-5, ITGA5 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.046ng/ml

Detection range: 0.1-40ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.046ng/ml

Detection range: 0.1-40ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Integrin Alpha-5

Code

E5482Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.046ng/ml

Detection range

0.1-40ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Integrin alpha-5/beta-1 (ITGA5:ITGB1) is a receptor for fibronectin and fibrinogen. It recognizes the sequence R-G-D in its ligands. ITGA5:ITGB1 binds to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1 (PubMed: 18635536, PubMed: 25398877). ITGA5:ITGB1 acts as a receptor for fibrillin-1 (FBN1) and mediates R-G-D-dependent cell adhesion to FBN1 (PubMed: 12807887, PubMed: 17158881). ITGA5:ITGB1 is a receptor for IL1B and binding is essential for IL1B signaling (PubMed: 29030430). ITGA5:ITGB3 is a receptor for soluble CD40LG and is required for CD40/CD40LG signaling (PubMed: 31331973). (Microbial infection) Integrin ITGA5:ITGB1 acts as a receptor for Human metapneumovirus. (Microbial infection) Integrin ITGA2:ITGB1 acts as a receptor for Human parvovirus B19. (Microbial infection) In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.

UniProt accession

P08648

MASS（Da）

114,536

GeneID

3678

Synonyms

CD antigen CD49e;CD49 antigen-like family member E;Fibronectin receptor subunit alpha;Integrin alpha-5;Integrin alpha-5 heavy chain;Integrin alpha-5 light chain;Integrin alpha-F;ITGA 5;ITGA5;VLA-5

Gene names

ITGA5

Research area

Others

Target protein

ITGA5

Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Protocol data

These standard curves of E5482Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
24ng/ml	2.183	2.138	2.108
	2.094
12ng/ml	1.377	1.305	1.275
	1.233
6ng/ml	0.856	0.764	0.733
	0.672
3ng/ml	0.514	0.451	0.42
	0.387
1.5ng/ml	0.265	0.248	0.218
	0.232
0ng/ml	0.028	0.031	0
	0.033

Precision

We measured random samples of E5482Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	11	0.66	6
Intra-Assay	2	18	16.5	0.68	4.1
Intra-Assay	3	18	3.7	0.22	6

Protocols

> Protocol Booklet

References

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Associated products

Datasheet

Size

Code

Price

48T

E5482Hu

$320.00
96T

E5482Hu

$458.00

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Lead time: Within two weeks

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