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Home > Product > ELISA Kit > Human Krueppel-like Factor 4, KLF4 ELISA Kit

Human Krueppel-like Factor 4, KLF4 ELISA Kit

Human Krueppel-like Factor 4, KLF4 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 5.31ng/L

Detection range: 10-2000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 5.31ng/L

Detection range: 10-2000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Krueppel-Like Factor 4

Code

E5546Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

5.31ng/L

Detection range

10-2000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Transcription factor; can act both as activator and as repressor. Binds the 5'-CACCC-3' core sequence. Binds to the promoter region of its own gene and can activate its own transcription. Regulates the expression of key transcription factors during embryonic development. Plays an important role in maintaining embryonic stem cells, and in preventing their differentiation. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development. Contributes to the down-regulation of p53/TP53 transcription.

UniProt accession

MASS(Da)

54,671

GeneID

9314

Synonyms

Epithelial zinc finger protein EZF;Gut-enriched krueppel-like factor;KLF 4;KLF4;Krueppel-like factor 4

Gene names

KLF4

Research area

Epigenetics and Nuclear Signaling

Target protein

KLF4

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E5546Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1280ng/L 1.752 1.718 1.669
1.685
640ng/L 1.075 1.081 1.033
1.088
320ng/L 0.443 0.438 0.389
0.432
160ng/L 0.156 0.186 0.137
0.216
80ng/L 0.126 0.125 0.076
0.124
0ng/L 0.05 0.049 0
0.047

Precision

We measured random samples of E5546Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 711.5 33.36 4.7
Intra-Assay 2 18 1411 111.78 7.9
Intra-Assay 3 18 298.6 7.78 2.6

Protocols

References

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 48T

    E5546Hu

    $320.00

  • 96T

    E5546Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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