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Home > Product > ELISA Kit > Human M-phase Inducer Phosphatase 2, CDC25B ELISA Kit

Human M-phase Inducer Phosphatase 2, CDC25B ELISA Kit

Human M-phase Inducer Phosphatase 2, CDC25B ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 9.76ng/L

Detection range: 20-3500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 9.76ng/L

Detection range: 20-3500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

M-Phase Inducer Phosphatase 2

Code

E5692Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

9.76ng/L

Detection range

20-3500ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Directly dephosphorylates CDK1 and stimulates its kinase activity. The three isoforms seem to have a different level of activity.

UniProt accession

MASS(Da)

64,987

GeneID

994

Synonyms

CDC 25B;CDC25B;Dual specificity phosphatase Cdc25B;M-phase inducer phosphatase 2

Gene names

CDC25B

Research area

Cell Biology

Target protein

CDC25B

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E5692Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
2000ng/L 1.692 1.706 1.654
1.719
1000ng/L 1.075 1.094 1.042
1.113
500ng/L 0.501 0.497 0.445
0.492
250ng/L 0.232 0.232 0.18
0.231
125ng/L 0.118 0.099 0.047
0.08
0ng/L 0.049 0.052 0
0.054

Precision

We measured random samples of E5692Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 550.6 21.99 4
Intra-Assay 2 18 920.4 41.44 4.5
Intra-Assay 3 18 1933.2 128.38 6.6

Protocols

References

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 48T

    E5692Hu

    $320.00

  • 96T

    E5692Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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