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Home > Product > ELISA Kit > Human Nuclear Factor of Activated T-cells 5, NFAT5 ELISA Kit

Human Nuclear Factor of Activated T-cells 5, NFAT5 ELISA Kit

Human Nuclear Factor of Activated T-cells 5, NFAT5 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 9.09ng/L

Detection range: 20-4500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 9.09ng/L

Detection range: 20-4500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Nuclear Factor of Activated T-Cells 5

Code

E5792Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

9.09ng/L

Detection range

20-4500ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Transcription factor involved, among others, in the transcriptional regulation of osmoprotective and inflammatory genes. Mediates the transcriptional response to hypertonicity (PubMed: 10051678). Positively regulates the transcription of LCN2 and S100A4 genes; optimal transactivation of these genes requires the presence of DDX5/DDX17 (PubMed: 22266867). Binds the DNA consensus sequence 5'-[ACT][AG]TGGAAA[CAT]A[TA][ATC][CA][ATG][GT][GAC][CG][CT]-3' (PubMed: 10377394).

UniProt accession

MASS(Da)

165,763

GeneID

10725

Synonyms

NFAT 5;NFAT5;NF-AT5;Nuclear factor of activated T-cells 5;T-cell transcription factor NFAT5;TonE-binding protein;TonEBP;Tonicity-responsive enhancer-binding protein

Gene names

NFAT5

Research area

Immunology

Target protein

NFAT5

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E5792Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
2400ng/L 2.65 2.581 2.536
2.513
1200ng/L 1.472 1.431 1.386
1.39
600ng/L 0.807 0.795 0.75
0.784
300ng/L 0.517 0.525 0.48
0.533
150ng/L 0.202 0.22 0.176
0.239
0ng/L 0.043 0.045 0
0.046

Precision

We measured random samples of E5792Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 1207.8 22.18 1.8
Intra-Assay 2 18 997.2 52.04 5.2
Intra-Assay 3 18 519.4 28.42 5.5

Protocols

References

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 48T

    E5792Hu

    $320.00

  • 96T

    E5792Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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