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Home > Product > ELISA Kit > Human Pericentriolar Material 1 Protein, PCM1 ELISA Kit

Human Pericentriolar Material 1 Protein, PCM1 ELISA Kit

Human Pericentriolar Material 1 Protein, PCM1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 4.49ng/L

Detection range: 10-2000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 4.49ng/L

Detection range: 10-2000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Pericentriolar Material 1 Protein

Code

E5847Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

4.49ng/L

Detection range

10-2000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Required for centrosome assembly and function (PubMed: 12403812, PubMed: 15659651, PubMed: 16943179). Essential for the correct localization of several centrosomal proteins including CEP250, CETN3, PCNT and NEK2 (PubMed: 12403812, PubMed: 15659651). Required to anchor microtubules to the centrosome (PubMed: 12403812, PubMed: 15659651). Involved in the biogenesis of cilia (PubMed: 20551181, PubMed: 24121310).

UniProt accession

MASS(Da)

228,544

GeneID

5108

Synonyms

hPCM-1;PCM 1;PCM1;PCM-1;Pericentriolar material 1 protein

Gene names

PCM1

Research area

Epigenetics and Nuclear Signaling

Target protein

PCM1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E5847Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1200ng/L 2.109 2.165 2.12
2.222
600ng/L 1.843 1.792 1.747
1.741
300ng/L 1.156 1.143 1.098
1.13
150ng/L 0.679 0.654 0.609
0.628
75ng/L 0.371 0.382 0.337
0.394
0ng/L 0.043 0.045 0
0.047

Precision

We measured random samples of E5847Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 486 28.27 5.8
Intra-Assay 2 18 272.6 18.13 6.7
Intra-Assay 3 18 151.9 7.94 5.2

Protocols

References

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 48T

    E5847Hu

    $320.00

  • 96T

    E5847Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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