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Home > Product > ELISA Kit > Human Ras-related Protein Rab-27A, RAB27A ELISA Kit

Human Ras-related Protein Rab-27A, RAB27A ELISA Kit

Human Ras-related Protein Rab-27A, RAB27A ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 8.94ng/L

Detection range: 20-3500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 8.94ng/L

Detection range: 20-3500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Ras-Related Protein Rab-27A

Code

E6059Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

8.94ng/L

Detection range

20-3500ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate homeostasis of late endocytic pathway, including endosomal positioning, maturation and secretion (PubMed: 30771381). Plays a role in cytotoxic granule exocytosis in lymphocytes. Required for both granule maturation and granule docking and priming at the immunologic synapse.

UniProt accession

MASS(Da)

24,868

GeneID

5873

Synonyms

GTP-binding protein Ram;RAB 27A;Rab-27;RAB27A;Ras-related protein Rab-27A

Gene names

RAB27A

Research area

Signal Transduction

Target protein

RAB27A

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E6059Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
2000ng/L 2.08 2.202 2.146
2.324
1000ng/L 1.718 1.711 1.656
1.704
500ng/L 1.02 1.026 0.97
1.032
250ng/L 0.664 0.703 0.648
0.743
125ng/L 0.47 0.461 0.405
0.452
0ng/L 0.063 0.055 0
0.048

Precision

We measured random samples of E6059Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 317.6 19.99 6.3
Intra-Assay 2 18 621.2 23.37 3.8
Intra-Assay 3 18 142.4 11.36 8

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E6059Hu

    $320.00

  • 96T

    E6059Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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