Tel:86 021 3100 7137

Publish and get free product : share with us your publications using BT LAB product and get free products.
Home > Product > ELISA Kit > Human Roundabout Homolog 1, ROBO1 ELISA Kit

Human Roundabout Homolog 1, ROBO1 ELISA Kit

Human Roundabout Homolog 1, ROBO1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.025ng/mL

Detection range: 0.05-20ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.025ng/mL

Detection range: 0.05-20ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Roundabout Homolog 1

Code

E6105Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.025ng/mL

Detection range

0.05-20ng/mL

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Receptor for SLIT1 and SLIT2 that mediates cellular responses to molecular guidance cues in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development (PubMed: 10102268, PubMed: 24560577). Interaction with the intracellular domain of FLRT3 mediates axon attraction towards cells expressing NTN1 (PubMed: 24560577). In axon growth cones, the silencing of the attractive effect of NTN1 by SLIT2 may require the formation of a ROBO1-DCC complex (By similarity). Plays a role in the regulation of cell migration via its interaction with MYO9B; inhibits MYO9B-mediated stimulation of RHOA GTPase activity, and thereby leads to increased levels of active, GTP-bound RHOA (PubMed: 26529257). May be required for lung development (By similarity).

UniProt accession

MASS(Da)

180,930

GeneID

6091

Synonyms

Deleted in U twenty twenty;H-Robo-1;ROBO 1;ROBO1;Roundabout homolog 1

Gene names

ROBO1

Research area

Cancer

Target protein

ROBO1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E6105Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
12ng/mL 2.174 2.197 2.141
2.219
6ng/mL 1.538 1.48 1.424
1.421
3ng/mL 0.916 0.921 0.865
0.926
1.5ng/mL 0.454 0.483 0.427
0.512
0.75ng/mL 0.275 0.259 0.203
0.243
0ng/mL 0.049 0.056 0
0.062

Precision

We measured random samples of E6105Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 6 0.23 3.9
Intra-Assay 2 18 2.4 0.12 4.9
Intra-Assay 3 18 3.2 0.18 5.8

Protocols

References

Customer reviews and Q&As

No reviews
No reviews

Associated products

  • Size

    Code

    Price

  • 48T

    E6105Hu

    $320.00

  • 96T

    E6105Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

Newsletter

Sign up

Newsletter

Sign up