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Home > Product > ELISA Kit > Human R-Spondin-2, RSPO2 ELISA Kit

Human R-Spondin-2, RSPO2 ELISA Kit

Human R-Spondin-2, RSPO2 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.026ng/mL

Detection range: 0.05-20ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.026ng/mL

Detection range: 0.05-20ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

R-Spondin-2

Code

E6108Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.026ng/mL

Detection range

0.05-20ng/mL

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Activator of the canonical Wnt signaling pathway by acting as a ligand for LGR4-6 receptors. Upon binding to LGR4-6 (LGR4, LGR5 or LGR6), LGR4-6 associate with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. Also regulates the canonical Wnt/beta-catenin-dependent pathway and non-canonical Wnt signaling by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway (PubMed: 21909076, PubMed: 21727895, PubMed: 22615920). During embryonic development, plays a crucial role in limb specification, amplifying the Wnt signaling pathway independently of LGR4-6 receptors, possibly by acting as a direct antagonistic ligand to RNF43 and ZNRF3, hence governing the number of limbs an embryo should form (PubMed: 29769720).

UniProt accession

MASS(Da)

28,315

GeneID

340419

Synonyms

hRspo2;Roof plate-specific spondin-2;RSPO 2;RSPO2;R-spondin-2

Gene names

RSPO2

Research area

Developmental Biology

Target protein

RSPO2

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E6108Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
12ng/mL 2.255 2.291 2.249
2.326
6ng/mL 1.481 1.581 1.539
1.682
3ng/mL 0.991 1.068 1.026
1.146
1.5ng/mL 0.78 0.715 0.673
0.65
0.75ng/mL 0.427 0.449 0.407
0.471
0ng/mL 0.036 0.042 0
0.048

Precision

We measured random samples of E6108Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E6108Hu

    $320.00

  • 96T

    E6108Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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