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Home > Product > ELISA Kit > Human Securin, PTTG1 ELISA Kit

Human Securin, PTTG1 ELISA Kit

Human Securin, PTTG1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 9.74ng/L

Detection range: 20-4500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 9.74ng/L

Detection range: 20-4500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Securin

Code

E6132Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

9.74ng/L

Detection range

20-4500ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Regulatory protein, which plays a central role in chromosome stability, in the p53/TP53 pathway, and DNA repair. Probably acts by blocking the action of key proteins. During the mitosis, it blocks Separase/ESPL1 function, preventing the proteolysis of the cohesin complex and the subsequent segregation of the chromosomes. At the onset of anaphase, it is ubiquitinated, conducting to its destruction and to the liberation of ESPL1. Its function is however not limited to a blocking activity, since it is required to activate ESPL1. Negatively regulates the transcriptional activity and related apoptosis activity of TP53. The negative regulation of TP53 may explain the strong transforming capability of the protein when it is overexpressed. May also play a role in DNA repair via its interaction with Ku, possibly by connecting DNA damage-response pathways with sister chromatid separation.

UniProt accession

MASS(Da)

22,024

GeneID

9232

Synonyms

Esp1-associated protein;hPTTG;Pituitary tumor-transforming gene 1 protein;PTTG 1;PTTG1;Securin;Tumor-transforming protein 1

Gene names

PTTG1

Research area

Cancer

Target protein

PTTG1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E6132Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
2400ng/L 2.079 2.128 2.067
2.176
1200ng/L 1.555 1.556 1.495
1.556
600ng/L 0.898 0.902 0.841
0.906
300ng/L 0.484 0.478 0.418
0.473
150ng/L 0.232 0.229 0.169
0.227
0ng/L 0.053 0.061 0
0.069

Precision

We measured random samples of E6132Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 301.1 15.31 5.1
Intra-Assay 2 18 746.7 41.03 5.5
Intra-Assay 3 18 1120 48.11 4.3

Protocols

References

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 48T

    E6132Hu

    $320.00

  • 96T

    E6132Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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