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Home > Product > ELISA Kit > Human Splicing Factor 3B Subunit 1, SF3B1 ELISA Kit

Human Splicing Factor 3B Subunit 1, SF3B1 ELISA Kit

Human Splicing Factor 3B Subunit 1, SF3B1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 9.97ng/L

Detection range: 20-6000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 9.97ng/L

Detection range: 20-6000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Splicing Factor 3B Subunit 1

Code

E6218Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

9.97ng/L

Detection range

20-6000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Involved in pre-mRNA splicing as a component of the splicing factor SF3B complex (PubMed: 27720643). SF3B complex is required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA (PubMed: 12234937). Together with other U2 snRNP complex components may also play a role in the selective processing of microRNAs (miRNAs) from the long primary miRNA transcript, pri-miR-17-92 (By similarity). May also be involved in the assembly of the 'E' complex (PubMed: 10882114). Belongs also to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron (PubMed: 15146077).

UniProt accession

MASS(Da)

145,830

GeneID

23451

Synonyms

Pre-mRNA-splicing factor SF3b 155 kDa subunit;SAP 155;SF3B1;SF3b155;Spliceosome-associated protein 155;Splicing factor 3B subunit 1

Gene names

SF3B1

Research area

Others

Target protein

SF3B1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E6218Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
3200ng/L 2.319 2.184 2.124
2.049
1600ng/L 1.441 1.382 1.322
1.324
800ng/L 0.819 0.825 0.765
0.83
400ng/L 0.439 0.444 0.384
0.449
200ng/L 0.266 0.267 0.207
0.268
0ng/L 0.053 0.06 0
0.067

Precision

We measured random samples of E6218Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 2367.6 105.32 4.4
Intra-Assay 2 18 1180.9 55.58 4.7
Intra-Assay 3 18 574 34.47 6

Protocols

References

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 48T

    E6218Hu

    $320.00

  • 96T

    E6218Hu

    $458.00

Add to Cart

Lead time: Within two weeks

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