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Home > Product > ELISA Kit > Human Tubulin Beta-3 Chain, TUBB3 ELISA Kit

Human Tubulin Beta-3 Chain, TUBB3 ELISA Kit

Human Tubulin Beta-3 Chain, TUBB3 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.146ng/ml

Detection range: 0.3-90ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.146ng/ml

Detection range: 0.3-90ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Tubulin Beta-3 Chain

Code

E6334Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.146ng/ml

Detection range

0.3-90ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. TUBB3 plays a critical role in proper axon guidance and mantainance. Binding of NTN1/Netrin-1 to its receptor UNC5C might cause dissociation of UNC5C from polymerized TUBB3 in microtubules and thereby lead to increased microtubule dynamics and axon repulsion (PubMed: 28483977). Plays a role in dorsal root ganglion axon projection towards the spinal cord (PubMed: 28483977).

UniProt accession

MASS(Da)

50,433

GeneID

10381

Synonyms

TUBB 3;TUBB3;Tubulin beta-3 chain;Tubulin beta-4 chain;Tubulin beta-III

Gene names

TUBB3

Research area

Signal Transduction

Target protein

TUBB3

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E6334Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
48ng/ml 2.25 2.126 2.079
2.002
24ng/ml 1.348 1.39 1.342
1.431
12ng/ml 0.767 0.747 0.699
0.727
6ng/ml 0.404 0.372 0.324
0.339
3ng/ml 0.144 0.148 0.101
0.152
0ng/ml 0.036 0.048 0
0.059

Precision

We measured random samples of E6334Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 13.5 0.58 4.3
Intra-Assay 2 18 35.1 1.46 4.2
Intra-Assay 3 18 10.5 0.49 4.7

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E6334Hu

    $320.00

  • 96T

    E6334Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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