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Home > Product > ELISA Kit > Human Zinc Finger Protein Gli1, GLI1 ELISA Kit

Human Zinc Finger Protein Gli1, GLI1 ELISA Kit

Human Zinc Finger Protein Gli1, GLI1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 7.85ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 7.85ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Zinc Finger Protein GLI1

Code

E6417Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

7.85ng/L

Detection range

15-3000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Acts as a transcriptional activator (PubMed: 19706761, PubMed: 10806483, PubMed: 19878745, PubMed: 24311597, PubMed: 24217340). Binds to the DNA consensus sequence 5'-GACCACCCA-3' (PubMed: 2105456, PubMed: 8378770, PubMed: 24217340). Regulates the transcription of specific genes during normal development (PubMed: 19706761). Plays a role in craniofacial development and digital development, as well as development of the central nervous system and gastrointestinal tract. Mediates SHH signaling (PubMed: 19706761, PubMed: 28973407). Plays a role in cell proliferation and differentiation via its role in SHH signaling (PubMed: 11238441, PubMed: 28973407). [Isoform 2]: Acts as a transcriptional activator, but activates a different set of genes than isoform 1. Activates expression of CD24, unlike isoform 1. Mediates SHH signaling. Promotes cancer cell migration.

UniProt accession

MASS(Da)

117,904

GeneID

2735

Synonyms

GLI 1;GLI1;Glioma-associated oncogene;Oncogene GLI;Zinc finger protein GLI1

Gene names

GLI1

Research area

Others

Target protein

GLI1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E6417Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1600ng/L 2.376 2.293 2.249
2.209
800ng/L 1.461 1.372 1.329
1.284
400ng/L 0.81 0.791 0.748
0.772
200ng/L 0.445 0.478 0.434
0.51
100ng/L 0.292 0.301 0.257
0.31
0ng/L 0.043 0.043 0
0.044

Precision

We measured random samples of E6417Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 176.7 11.85 6.7
Intra-Assay 2 18 962.3 35.09 3.6
Intra-Assay 3 18 487.6 29.42 6

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E6417Hu

    $320.00

  • 96T

    E6417Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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