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Home > Product > ELISA Kit > Human Gamma-aminobutyric Acid Type B Receptor Subunit 2, GABBR2 ELISA Kit

Human Gamma-aminobutyric Acid Type B Receptor Subunit 2, GABBR2 ELISA Kit

Human Gamma-aminobutyric Acid Type B Receptor Subunit 2, GABBR2 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 5.63ng/L

Detection range: 10-2000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 5.63ng/L

Detection range: 10-2000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Gamma-Aminobutyric Acid Type B Receptor Subunit 2

Code

E6458Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

5.63ng/L

Detection range

10-2000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Component of a heterodimeric G-protein coupled receptor for GABA, formed by GABBR1 and GABBR2 (PubMed: 9872316, PubMed: 9872744, PubMed: 15617512, PubMed: 18165688, PubMed: 22660477, PubMed: 24305054). Within the heterodimeric GABA receptor, only GABBR1 seems to bind agonists, while GABBR2 mediates coupling to G proteins (PubMed: 18165688). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase (PubMed: 10075644, PubMed: 10773016, PubMed: 24305054). Signaling inhibits adenylate cyclase, stimulates phospholipase A2, activates potassium Channels, inactivates voltage-dependent calcium-Channels and modulates inositol phospholipid hydrolysis (PubMed: 10075644, PubMed: 9872744, PubMed: 10906333, PubMed: 10773016). Plays a critical role in the fine-tuning of inhibitory synaptic transmission (PubMed: 9872744, PubMed: 22660477). Pre-synaptic GABA receptor inhibits neurotransmitter release by down-regulating high-voltage activated calcium Channels, whereas postsynaptic GABA receptor decreases neuronal excitability by activating a prominent inwardly rectifying potassium (Kir) conductance that underlies the late inhibitory postsynaptic potentials (PubMed: 9872316, PubMed: 10075644, PubMed: 9872744, PubMed: 22660477). Not only implicated in synaptic inhibition but also in hippocampal long-term potentiation, slow wave sleep, muscle relaxation and antinociception (Probable).

UniProt accession

MASS(Da)

105,821

GeneID

9568

Synonyms

GABA-B receptor 2;GABABR2;GABA-BR2;GABA-B-R2;GABBR 2;GABBR2;Gamma-aminobutyric acid type B receptor subunit 2;Gb2;G-protein coupled receptor 51;HG20

Gene names

GABBR2

Research area

Others

Target protein

GABBR2

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E6458Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1200ng/L 2.353 2.365 2.336
2.377
600ng/L 1.335 1.379 1.35
1.422
300ng/L 0.643 0.632 0.603
0.621
150ng/L 0.366 0.392 0.363
0.419
75ng/L 0.207 0.178 0.148
0.148
0ng/L 0.039 0.029 0
0.02

Precision

We measured random samples of E6458Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 747.4 21.63 2.9
Intra-Assay 2 18 327.6 12.95 4
Intra-Assay 3 18 159 7.49 4.7

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E6458Hu

    $320.00

  • 96T

    E6458Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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