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Home > Product > ELISA Kit > Human Perilipin-5, PLIN5 ELISA Kit

Human Perilipin-5, PLIN5 ELISA Kit

Human Perilipin-5, PLIN5 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 11.93ng/L

Detection range: 20-4500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 11.93ng/L

Detection range: 20-4500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Perilipin-5

Code

E6484Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

11.93ng/L

Detection range

20-4500ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Lipid droplet-associated protein that maintains the balance between lipogenesis and lipolysis and also regulates fatty acid oxidation in oxidative tissues. Recruits mitochondria to the surface of lipid droplets and is involved in lipid droplet homeostasis by regulating both the storage of fatty acids in the form of triglycerides and the release of fatty acids for mitochondrial fatty acid oxidation. In lipid droplet triacylglycerol hydrolysis, plays a role as a scaffolding protein for three major key lipolytic players: ABHD5, PNPLA2 and LIPE. Reduces the triacylglycerol hydrolase activity of PNPLA2 by recruiting and sequestering PNPLA2 to lipid droplets. Phosphorylation by PKA enables lipolysis probably by promoting release of ABHD5 from the perilipin scaffold and by facilitating interaction of ABHD5 with PNPLA2. Also increases lipolysis through interaction with LIPE and upon PKA-mediated phosphorylation of LIPE (By similarity).

UniProt accession

MASS(Da)

50,791

GeneID

440503

Synonyms

Lipid storage droplet protein 5;Perilipin-5;PLIN 5;PLIN5

Gene names

PLIN5

Research area

Cardiovascular

Target protein

PLIN5

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E6484Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
2400ng/L 2.295 2.269 2.217
2.243
1200ng/L 1.263 1.322 1.27
1.38
600ng/L 0.853 0.817 0.765
0.781
300ng/L 0.447 0.456 0.404
0.464
150ng/L 0.296 0.31 0.258
0.325
0ng/L 0.062 0.052 0
0.042

Precision

We measured random samples of E6484Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 351.3 25.35 7.2
Intra-Assay 2 18 848.7 53.92 6.4
Intra-Assay 3 18 1296.6 40.33 3.1

Protocols

References

Customer reviews and Q&As

No reviews
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Associated products

  • Size

    Code

    Price

  • 48T

    E6484Hu

    $320.00

  • 96T

    E6484Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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