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Home > Product > ELISA Kit > Human Cartilage Intermediate Layer Protein 1, CILP ELISA Kit

Human Cartilage Intermediate Layer Protein 1, CILP ELISA Kit

Human Cartilage Intermediate Layer Protein 1, CILP ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 26.34ng/L

Detection range: 50-10000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 26.34ng/L

Detection range: 50-10000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Cartilage Intermediate Layer Protein 1

Code

E6610Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

26.34ng/L

Detection range

50-10000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Probably plays a role in cartilage scaffolding. May act by antagonizing TGF-beta1 (TGFB1) and IGF1 functions. Has the ability to suppress IGF1-induced proliferation and sulfated proteoglycan synthesis, and inhibits ligand-induced IGF1R autophosphorylation. May inhibit TGFB1-mediated induction of cartilage matrix genes via its interaction with TGFB1. Overexpression may lead to impair chondrocyte growth and matrix repair and indirectly promote inorganic pyrophosphate (PPi) supersaturation in aging and osteoarthritis cartilage.

UniProt accession

MASS(Da)

132,565

GeneID

8483

Synonyms

Cartilage intermediate layer protein 1;Cartilage intermediate layer protein 1 C1;Cartilage intermediate layer protein 1 C2;Cartilage intermediate-layer protein;CILP;CILP-1

Gene names

CILP

Research area

Signal Transduction

Target protein

CILP

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E6610Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
6400ng/L 1.869 1.92 1.877
1.971
3200ng/L 1.154 1.03 0.987
0.906
1600ng/L 0.609 0.517 0.474
0.424
800ng/L 0.212 0.252 0.209
0.293
400ng/L 0.077 0.114 0.071
0.15
0ng/L 0.037 0.043 0
0.048

Precision

We measured random samples of E6610Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 2041.9 104.36 5.1
Intra-Assay 2 18 3509 164.79 4.7
Intra-Assay 3 18 4443.3 106.55 2.4

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E6610Hu

    $320.00

  • 96T

    E6610Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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