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Home > Product > ELISA Kit > Human Ephrin B1, EFNB1 ELISA Kit

Human Ephrin B1, EFNB1 ELISA Kit

Human Ephrin B1, EFNB1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.031ng/ml

Detection range: 0.05-30ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.031ng/ml

Detection range: 0.05-30ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Ephrin B1

Code

E6628Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.031ng/ml

Detection range

0.05-30ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Cell surface transmembrane ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development (PubMed: 8070404, PubMed: 7973638). Binding to Eph receptors residing on adjacent cells leads to contact-dependent bidirectional signaling into neighboring cells (PubMed: 8070404, PubMed: 7973638). Shows high affinity for the receptor tyrosine kinase EPHB1/ELK (PubMed: 8070404, PubMed: 7973638). Can also bind EPHB2 and EPHB3 (PubMed: 8070404). Binds to, and induces collapse of, commissural axons/growth cones in vitro (By similarity). May play a role in constraining the orientation of longitudinally projecting axons (By similarity).

UniProt accession

MASS(Da)

38,007

GeneID

1947

Synonyms

EFL-3;EFNB 1;EFNB1;ELK ligand;ELK-L;EPH-related receptor tyrosine kinase ligand 2;Ephrin-B1;Ephrin-B1 C-terminal fragment;Ephrin-B1 CTF;Ephrin-B1 ICD;Ephrin-B1 intracellular domain;LERK-2

Gene names

EFNB1

Research area

Others

Target protein

EFNB1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E6628Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
16ng/ml 2.656 2.584 2.539
2.513
8ng/ml 1.559 1.506 1.46
1.453
4ng/ml 0.878 0.905 0.859
0.932
2ng/ml 0.523 0.45 0.405
0.377
1ng/ml 0.206 0.208 0.163
0.211
0ng/ml 0.054 0.046 0
0.037

Precision

We measured random samples of E6628Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 5.7 0.34 6
Intra-Assay 2 18 7.1 0.25 3.4
Intra-Assay 3 18 3.3 0.18 5.4

Protocols

References (1)

Role of CNK1, Ephrin B1, GPR19 and SMURF1 in breast cancer early diagnosis, metastasis and drug resistance

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 48T

    E6628Hu

    $320.00

  • 96T

    E6628Hu

    $458.00

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Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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