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Home > Product > ELISA Kit > Human Atp-binding Cassette Sub-family G Member 8, ABCG8 ELISA Kit

Human Atp-binding Cassette Sub-family G Member 8, ABCG8 ELISA Kit

Human Atp-binding Cassette Sub-family G Member 8, ABCG8 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 12.63ng/L

Detection range: 20-7000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 12.63ng/L

Detection range: 20-7000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Atp-Binding Cassette Sub-Family G Member 8

Code

E6711Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

12.63ng/L

Detection range

20-7000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

ABCG5 and ABCG8 form an obligate heterodimer that mediates Mg(2+)- and ATP-dependent sterol transport across the cell membrane. Plays an essential role in the selective transport of the dietary cholesterol in and out of the enterocytes and in the selective sterol excretion by the liver into bile (PubMed: 11099417, PubMed: 11452359, PubMed: 27144356, PubMed: 15054092). Required for normal sterol homeostasis (PubMed: 11099417, PubMed: 11452359, PubMed: 15054092). The heterodimer with ABCG5 has ATPase activity (PubMed: 16893193, PubMed: 20210363, PubMed: 27144356).

UniProt accession

MASS(Da)

75,679

GeneID

64241

Synonyms

ABCG 8;ABCG8;ATP-binding cassette sub-family G member 8;Sterolin-2

Gene names

ABCG8

Research area

Cardiovascular

Target protein

ABCG8

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E6711Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
4000ng/L 2.395 2.356 2.318
2.318
2000ng/L 1.232 1.287 1.249
1.341
1000ng/L 0.765 0.694 0.656
0.623
500ng/L 0.313 0.303 0.265
0.293
250ng/L 0.158 0.184 0.146
0.21
0ng/L 0.034 0.038 0
0.042

Precision

We measured random samples of E6711Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 1068 63.03 5.9
Intra-Assay 2 18 2466 80.3 3.3
Intra-Assay 3 18 1351.5 61.67 4.6

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E6711Hu

    $320.00

  • 96T

    E6711Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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