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Home > Product > ELISA Kit > Human Eukaryotic Elongation Factor 2 Kinase, EEF2K ELISA Kit

Human Eukaryotic Elongation Factor 2 Kinase, EEF2K ELISA Kit

Human Eukaryotic Elongation Factor 2 Kinase, EEF2K ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 9.94pg/ml

Detection range: 20-4500pg/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 9.94pg/ml

Detection range: 20-4500pg/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Eukaryotic Elongation Factor 2 Kinase

Code

E6821Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

9.94pg/ml

Detection range

20-4500pg/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Threonine kinase that regulates protein synthesis by controlling the rate of Peptide chain elongation. Upon activation by a variety of upstream kinases including AMPK or TRPM7, phosphorylates the elongation factor EEF2 at a single site, renders it unable to bind ribosomes and thus inactive. In turn, the rate of protein synthesis is reduced.

UniProt accession

MASS(Da)

82,144

GeneID

29904

Synonyms

Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase;eEF-2 kinase;EEF2K;eEF-2K;Eukaryotic elongation factor 2 kinase

Gene names

EEF2K

Research area

Epigenetics and Nuclear Signaling

Target protein

EEF2K

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E6821Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
2400pg/ml 2.363 2.44 2.382
2.517
1200pg/ml 1.382 1.373 1.315
1.363
600pg/ml 0.739 0.794 0.736
0.85
300pg/ml 0.426 0.426 0.368
0.427
150pg/ml 0.227 0.231 0.173
0.235
0pg/ml 0.054 0.058 0
0.062

Precision

We measured random samples of E6821Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 1131.5 33.07 2.9
Intra-Assay 2 18 335.3 18.06 5.4
Intra-Assay 3 18 659.3 36.25 5.5

Protocols

References

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 48T

    E6821Hu

    $320.00

  • 96T

    E6821Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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