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Home > Product > ELISA Kit > Human Protein Phosphatase 2A, PP2A ELISA Kit

Human Protein Phosphatase 2A, PP2A ELISA Kit

Human Protein Phosphatase 2A, PP2A ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.018ng/ml

Detection range: 0.05-10ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.018ng/ml

Detection range: 0.05-10ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Protein Phosphatase 2A

Code

E6870Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.018ng/ml

Detection range

0.05-10ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Enzyme with a broad specificity. Negatively regulates TGF-beta signaling through dephosphorylating SMAD2 and SMAD3, resulting in their dissociation from SMAD4, nuclear export of the SMADs and termination of the TGF-beta-mediated signaling. Dephosphorylates PRKAA1 and PRKAA2. Plays an important role in the termination of TNF-alpha-mediated NF-kappa-B activation through dephosphorylating and inactivating IKBKB/IKKB.

UniProt accession

MASS(Da)

42,448

GeneID

5494

Synonyms

PP2C-alpha;PPM 1A;PPM1A;Protein phosphatase 1A;Protein phosphatase 2C isoform alpha;Protein phosphatase IA

Gene names

PPM1A

Research area

Others

Target protein

PPM1A

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E6870Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
6.4ng/ml 2.57 2.556 2.519
2.541
3.2ng/ml 1.482 1.417 1.38
1.351
1.6ng/ml 0.936 0.873 0.836
0.809
0.8ng/ml 0.427 0.473 0.436
0.518
0.4ng/ml 0.271 0.234 0.197
0.197
0ng/ml 0.028 0.037 0
0.045

Precision

We measured random samples of E6870Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 3.2 0.1 3.2
Intra-Assay 2 18 1.6 0.1 6.2
Intra-Assay 3 18 1.3 0.08 6.6

Protocols

References

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 48T

    E6870Hu

    $320.00

  • 96T

    E6870Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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