Tel:86 021 3100 7137

Publish and get free product : share with us your publications using BT LAB product and get free products.
Home > Product > ELISA Kit > Human Eukaryotic Translation Initiation Factor 2-alpha Kinase 3, EIF2AK3 ELISA Kit

Human Eukaryotic Translation Initiation Factor 2-alpha Kinase 3, EIF2AK3 ELISA Kit

Human Eukaryotic Translation Initiation Factor 2-alpha Kinase 3, EIF2AK3 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.019ng/ml

Detection range: 0.05-15ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.019ng/ml

Detection range: 0.05-15ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Eukaryotic Translation Initiation Factor 2-Alpha Kinase 3,Eif2Ak3,

Code

E7149Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.019ng/ml

Detection range

0.05-15ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Metabolic-stress sensing protein kinase that phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 (EIF2S1/eIF-2-alpha) in response to various stress conditions. Key activator of the integrated stress response (ISR) required for adaptation to various stress, such as unfolded protein response (UPR) and low amino acid availability. EIF2S1/eIF-2-alpha phosphorylation in response to stress converts EIF2S1/eIF-2-alpha in a global protein synthesis inhibitor, leading to a global attenuation of cap-dependent translation, while concomitantly initiating the preferential translation of ISR-specific mRNAs, such as the transcriptional activator ATF4, and hence allowing ATF4-mediated reprogramming. Serves as a critical effector of unfolded protein response (UPR)-induced G1 growth arrest due to the loss of cyclin-D1 (CCND1). Involved in control of mitochondrial morphology and function.

UniProt accession

MASS(Da)

125,216

GeneID

9451

Synonyms

EIF2AK3;Eukaryotic translation initiation factor 2-alpha kinase 3;HsPEK;Pancreatic eIF2-alpha kinase;PRKR-like endoplasmic reticulum kinase

Gene names

EIF2AK3

Research area

Others

Target protein

EIF2AK3

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E7149Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
8ng/ml 2.373 2.213 2.157
2.053
4ng/ml 1.73 1.794 1.738
1.858
2ng/ml 1.155 1.179 1.123
1.203
1ng/ml 0.687 0.694 0.638
0.701
0.5ng/ml 0.352 0.367 0.311
0.382
0ng/ml 0.062 0.056 0
0.05

Precision

We measured random samples of E7149Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 3.1 0.16 5.2
Intra-Assay 2 18 2 0.13 6.8
Intra-Assay 3 18 1 0.06 5.7

Protocols

References (1)

A Pilot Study on Blood Components in COVID-19 Affected Subjects: A Correlation to UPR Signalling and ER-Stress

Customer reviews and Q&As

No reviews
No reviews

Associated products

  • Size

    Code

    Price

  • 48T

    E7149Hu

    $320.00

  • 96T

    E7149Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

Newsletter

Sign up

Newsletter

Sign up